_____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform pore size than that of agar. It is also easily poured and does not denature the samples. In agarose gel electrophoresis‚ DNA or RNA fragments are separated or isolated according
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Anjali Patel Riley Egan‚ Nick Morrison 10/2/13 Block 2 LAB REPORT OUTLINE FOR DIFFUSION/OSMOSIS LAB Introduction: Diffusion is the net movement of small or uncharged molecules or substances from areas of higher concentration to areas of lower concentration. There is simple diffusion‚ as explained‚ and facilitated diffusion. Facilitated diffusion‚ on the other hand‚ involves larger or charged molecules or substances that need a protein tunnel through the membrane to help the substance enter and
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simple diffusion and understand how to make % solutions. Exercise 2 demonstrated the principle of osmosis: observing the movement of water by osmosis from an area of lower concentration of a solute to an area of higher concentration of a solute. Materials and Method: Refer to pages 47-51 of Bio 203L Lab Manual. Results/ Discussion: The effect of the chloride ion on its rate of diffusion in agar means it will diffuse faster because it is more concentrated. We graphed the diffusion of chloride
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The effect of molecular weight on the diffusion rates of Potassium permanganate (KMnO4)‚ Potassium dichromate(K2Cr2O7) and Methylene Blue^1 _________________ Group 3 Sec. X-1L February 6‚ 2012 ____________________ ABSTRACT The effect of molecular weight on the diffusion of substances were tested through the use of agar-water gel with three wells placed in a petri dish. Drops of three different substances namely: Potassium
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Making Agar Cushions 1. Start with wearing gloves and swabbing them with 70% ethanol to keep the materials that will be used in the experiment sterile. 2. Wipe the work station with Wescodyne and paper towels. It is important to keep the sterile materials such as slides in petri dish‚ Pasteur pipette in container and forceps covered as much as possible. 3. Place four to five filter papers in the petri dish and drip few drops of water on the strips‚ this will maintain moisture in the agar cushions
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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For this experiment you will need 39 agar plates‚ sterile cotton swabs‚ a concentrated amount of Escherichia coli (E. coli)‚ three different levels of antibiotic (Ampicillin: 1mg‚ 5mg‚ and 10mg)‚ a bunsen burner‚ metal tweezers‚ an incubator‚ 27 broths‚ and a ruler which should all be provided through your laboratory. For the first generation‚ you will be using 12 plates. Three of the plates will be labeled G1 control‚ three of the plates will be labeled G1/1 mg‚ three plates will be labeled G1/5
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‘Agar extract as additive component in making floor wax’ Jericho Earl F. Follosco Clifford Cyril R. Jumawan Abul Ghaffar A Lintasan (Researchers) Dr. Estela Florentino (Researchers Teacher) B. Statement of the Problem This study aims to produce an agar extract as additive component in making floor wax. Specifically it sought to answer the following questions: a.) Which of the following concentrations
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Factors Affecting Diffusion How does temperature affect diffusion? What is Diffusion? Definition 1: Diffusion is a process where by molecules move from an area of high concentration area to an area of low concentration. Definition 2: Diffusion is an evidence for moving particles. The spreading out of a gas is called diffusion and it takes place in haphazard and random way. Gases diffuse because the particles collide with other particles and bounce of in all directions. For example when
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Materials and Methods: Inoculation of the unknown was done on a nutrient slant agar The nutrient agar was then incubated at 27°C for one week. The agar appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain
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