References: 2. National Committee for Clinical Laboratory Standards (NCCLS)‚ Disk Diffusion Supplemental Tables. NCCLS Document M100-S13 (M2)‚ Wayne‚ PA‚ 2003. 3. Bangroo A.K.‚ Ramji K.‚ Smita C.: Honey dressing in paediatric burns. J. Indian Association Paediatric Surgeons‚ 10: 172-5‚ 2005. 4. Molan P.C.: Honey as a topical antibacterial
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be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen. Hypothesis: For this experiment‚ antibodies will be placed in wells on an agar plate‚ surrounded by wells containing serum (proteins‚ antigens etc.). If the antibody interacts with the antigen of surrounding wells‚ then the presence
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Equipment: * 3 agar plates * Blank discs * Antibiotic assay disc * Culture of Bacillus subtilis * Forceps * Bunsen Burner * Inoculating loop * Tea Tree oil * Heat Mat Method: First Agar Plate * Sterilise inoculating loop using Bunsen burner‚ allow inoculating loop to cool before using inoculating loop to pick up‚ the liquid of the culture. * Agar plate to be open as little as possible‚ minimising
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An agar plate is a Petri dish that contains a combination of agar and nutrients that help microorganisms grow. The proper method of setting microorganisms on an agar plate is know as “streaking”. In order to streak‚ the microorganisms are placed on a sterile swab or metal wire‚ which is then dragged lightly against the agar solution‚ leaving behind the microorganisms. The amount of organisms is greatest at the beginning
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investigation of the effect of fresh pineapple on the setting of jelly Propose a hypothesis (p. 1-2) It is the fresh pineapple that causes the jelly to remain in liquid form. Design and perform an experiment (p. 1-2) 1 (Answer varies with Ss. The recommended quantity of jelly powder and water is stated on the packet of the jelly powder. Jelly will not set if it is too dilute.) 2 Method II. This makes sure the concentrations of the jelly solutions in different containers are the same. A Identifying
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Lab Report T 1:35 Prof. Clemente Fernandez MCB2010L 11/19/13 Cristina Carvajal Fatima Hussain Nordia Johnson Jessica Rignack Amoeba: It is a unicellular protozoan that lacks a definite shape. Live in both fresh water and marine habitats. Some are found in soil‚ and many have adapted to parasitic life on the body of marine animals or internal organs of both aquatic and terrestrial animals. Amoeba reproduces asexually by binary and multiple fissions. They eat algae‚ bacteria‚ other
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aureus were maintained on Tryptic Soy agar (TSA)‚ following incubation at 37 oC for 24 h. Inocula were obtained from overnight fresh cultures adjusted to approximately log 108 CFU/ml‚ a turbidity equivalent to a 0.5 McFarland standard (Kroning et al.‚ 2016). The inocula were spread on the surface of Mueller-Hinton agar (MHA) plates. The disk diffusion test‚ recommended by the Clinical and Laboratory Standards Institute (CLSI‚ 2015)‚ was employed
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The unusual members of NFGNB represent a challenge for the routine clinical microbiological laboratories‚ owing to their complex identification along with their unpredictable antimicrobial susceptibility patterns 14. The isolation rate of NFGNB (18.7%) recorded in our study comes in agreement with a previous Indian study carried out in a tertiary care hospital‚ where it was 16.18% 15. Whereas‚ it comes higher the result recorded in another Indian tertiary care hospital where it was 9.32% 16. The
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effectiveness of an antimicrobial agent against bacteria grown in culture. This is called the Kirby-Bauer disk-diffusion method‚ and here is how it works. The bacteria of interest are swabbed uniformly across a culture plate. Then a filter-paper disk‚ impregnated with the compound to be tested‚ is placed on the surface of the agar. The compound diffuses out from the filter paper into the agar. The concentration of the compound will be higher next to the disk‚ and will decrease gradually as distance
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fungus grows. Therefore optimum temperature will increase growth. The diffusion rate of these dead organic matters is affected by temperature. According to Kinetic theory higher temperatures increase the energy and therefore the movement of the molecules‚ increasing the rate of diffusion‚ therefore increasing growth. Lower temperatures decrease the energy of the molecules‚ thus decreasing the rate of diffusion. The optimum diffusion rate varies with different fungi’s. The growth of Aspergillus oryzae
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