An agar plate is a Petri dish that contains a combination of agar and nutrients that help microorganisms grow. The proper method of setting microorganisms on an agar plate is know as “streaking”. In order to streak‚ the microorganisms are placed on a sterile swab or metal wire‚ which is then dragged lightly against the agar solution‚ leaving behind the microorganisms. The amount of organisms is greatest at the beginning
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investigation of the effect of fresh pineapple on the setting of jelly Propose a hypothesis (p. 1-2) It is the fresh pineapple that causes the jelly to remain in liquid form. Design and perform an experiment (p. 1-2) 1 (Answer varies with Ss. The recommended quantity of jelly powder and water is stated on the packet of the jelly powder. Jelly will not set if it is too dilute.) 2 Method II. This makes sure the concentrations of the jelly solutions in different containers are the same. A Identifying
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The unusual members of NFGNB represent a challenge for the routine clinical microbiological laboratories‚ owing to their complex identification along with their unpredictable antimicrobial susceptibility patterns 14. The isolation rate of NFGNB (18.7%) recorded in our study comes in agreement with a previous Indian study carried out in a tertiary care hospital‚ where it was 16.18% 15. Whereas‚ it comes higher the result recorded in another Indian tertiary care hospital where it was 9.32% 16. The
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aureus were maintained on Tryptic Soy agar (TSA)‚ following incubation at 37 oC for 24 h. Inocula were obtained from overnight fresh cultures adjusted to approximately log 108 CFU/ml‚ a turbidity equivalent to a 0.5 McFarland standard (Kroning et al.‚ 2016). The inocula were spread on the surface of Mueller-Hinton agar (MHA) plates. The disk diffusion test‚ recommended by the Clinical and Laboratory Standards Institute (CLSI‚ 2015)‚ was employed
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Lab Report T 1:35 Prof. Clemente Fernandez MCB2010L 11/19/13 Cristina Carvajal Fatima Hussain Nordia Johnson Jessica Rignack Amoeba: It is a unicellular protozoan that lacks a definite shape. Live in both fresh water and marine habitats. Some are found in soil‚ and many have adapted to parasitic life on the body of marine animals or internal organs of both aquatic and terrestrial animals. Amoeba reproduces asexually by binary and multiple fissions. They eat algae‚ bacteria‚ other
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effectiveness of an antimicrobial agent against bacteria grown in culture. This is called the Kirby-Bauer disk-diffusion method‚ and here is how it works. The bacteria of interest are swabbed uniformly across a culture plate. Then a filter-paper disk‚ impregnated with the compound to be tested‚ is placed on the surface of the agar. The compound diffuses out from the filter paper into the agar. The concentration of the compound will be higher next to the disk‚ and will decrease gradually as distance
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fungus grows. Therefore optimum temperature will increase growth. The diffusion rate of these dead organic matters is affected by temperature. According to Kinetic theory higher temperatures increase the energy and therefore the movement of the molecules‚ increasing the rate of diffusion‚ therefore increasing growth. Lower temperatures decrease the energy of the molecules‚ thus decreasing the rate of diffusion. The optimum diffusion rate varies with different fungi’s. The growth of Aspergillus oryzae
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Hypothesis: The hypothesis for this particular practical experiment is that‚ as the pH decreases or increases from neutral pH of approximately 7‚ the amount of microbial growth on the agar solution will decrease Materials: Agar Solution Preparation: *hand sanitizer/ hand wash soap *small container *1g of agar powder *0.25g of beef extract stock *60mL of heated water *electronic balance *small spoons (2x) *teaspoons (2x) *0.2M Acetic Acid Solution (pH of 3) *0.2M Sodium Hydroxide Solution
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recorded and the height. * Diffusion in solids -4 agar plates -KMnO4 crystals -methyl orange -refrigeration -ruler The experiment requires 4 plates of agar due to his transparency and the colloid that forms when mixed with water. One pair of plates is labeled 4^0 C and the second pair RT (room temperature). After taking the lids of the first pair ‚using a tooth pick‚ a small crystal of potassium permanganate is placed in the center of the of the agar and the same amount of methyl
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Antibacterial activity of allopathic medicine versus phytomedicine against Enterococcus faecalis: An in-vitro study. Abstract Background: A successful endodontic treatment depends on appropriate instrumentation‚ irrigation‚ decontamination and obturation. Decontamination of root canal plays a crucial role in the success of endodontic treatment. Therefore‚ a suitable medicament is essential which can effectively eliminate bacterial contamination. Aim and objectives: To investigate and compare the
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