have a greater surface area than spheres of the same volume. In Part B‚ the relationship between diffusion and surface area to volume ratio was investigated. Three agar-phenolphthalein-sodium hydroxide cubes of different sizes were exposed to a sulfuric acid solution. It was found that the cube with the smallest volume had the highest rate of diffusion. It was concluded that since life-preserving diffusion is more efficient for small cells‚ size influences growth and
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Petri dish- This is used to transfer the agar cubes as you should put the cubes in there as some of the chemicals can be dangerous Safety goggles- These are essential to keep your eyes protected from harmful chemicals White tile- To help to see the colour change Stop clock- To time how long it takes for the cube to change colour. Method 1-Stop the clock and quickly but carefully place one of the agar cubes into the specimen tube containing 3.7g/100cm3 of hydrochloric acid
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The agar diffusion study of steam distilled ethanolic extract of aerial parts of the plant shows strong inhibition of plant pathogens‚ Rhizoctonia sp. Botryosphaeria rhodina and two strains of Alternaria sp. (Alternaria sp. (A1) and Alternaria sp. (A2) - tomato wilt fungi) which was mainly due to presence of fungitoxic phenolic compound eugenol. When this eugenol was further subjected to agar diffusion assay it shows strong efficacy against Alternaria
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sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate. 5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate‚ making sure the lid of the culture container does not touch the surface‚ and that the lid of the agar plate is quickly replaced after this step has been carried out. 6. Sterilise the grabbing part and up the handle
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Cnidaria Hydra‚ Jellyfish‚ Coral‚ & Sea Anemones Phylum Cnidaria- Examples Sea Anemones and Corals - Anthozoa Hydra- Hydrozoa Jellyfish - Scyphozoa Ctenophores - non stinging Cnidarians; Comb Jelly Anatomy A. Polymorphism- Cnidarians have more than one body form: 1.Polyp 2. Medusa Polyp Form Tube with tentacles around the mouth Sessile Medusa Form Umbrella Shape Tentacles around mouth Motile‚ Free-swimming Tentacles Have nematocysts (stinging cells) coiled thread discharges
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will become hypotonic and swell up as the time increased. “Diffusion is a passive process in which the random mixing of particles in a solution occurs because of the particle ’s kinetic energy “(Allen C. 2006)‚ and is subjected to detect the presence of each of the substances given in the biochemical testing‚ the increase in temperature‚ and different reagent used are some aspect that could cause the difference of diffusion in certain solution. It was clear that the protocols Glucose‚ Albumin
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Cell Biology- Osmosis‚ Cell Size and Diffusion and Enzymes 1.0 INTRODUCTION Cells are the basic building blocks of all living things. They provide structure for the body‚ take in nutrients from the food‚ convert those nutrients into energy‚ and carry out specialized functions. Cells also contain the body’s genetic material and can make copies of themselves. A cell is also a metabolic compartment where many different chemical reaction occur. There are two types of cells‚ eukaryotic and prokaryotic
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test‚ such as the Kirby Bauer Method‚ are used to determine susceptibility to various antimicrobials. Why are they not used to determine resistance? a. They grow a resistance to antibiotics and antimicrobials. Kirby Bauer is used to determine diffusion of a disk‚ to measure the effectiveness of antibiotics towards pathogenic microbes. 4. Compare and contrast the following terms: antiseptic‚ disinfectant‚ sterilant. Provide one example for each of the terms. a. Antiseptic is used on living
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RESEARCH PAPER ON BIOLOGICAL ACTIVITY OF ACTINOMYCETES ................ ABSTRACT: The microorganisms are ubiquitous in nature. They are found in probably every environmental condition present on earth. Actinomycetes were originally considered to be an intermediate group between bacteria and fungi but now are recognized as prokaryotic organisms with high G+C (>55%) content in their DNA. Our project aim is to evaluate some biological activities of Actinomycetes like:
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This lab has brought great interest to me because this is the way physicians’ control/treat infectious disease within our bodies. In this lab‚ chemotherapeutic agents were evaluated by the disk-diffusion method. Chemotherapeutic agents are placed on the surface of a Petri plate containing Mueller-Hinton agar‚ which allows the agents to diffuse freely‚ and a growth medium is incubated over its’ surface. During incubation‚ the chemotherapeutic agents move from levels of high concentration‚ to low levels
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