Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
Premium Bacteria Metabolism Enzyme
Staphylococcus aureus‚ were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies. Resulting data was analyzed to provide understanding of the use of differing culture media and conditions for bacterial growth. RESULTSFour different agar types were used in this experiment. The first (Nutrient) allowed for growth of both E. coli and S. aureus. The second agar used (MKL) inhibited the growth of S. aureus but allowed the growth of E. coli
Premium Bacteria Microbiology Staphylococcus
PATTERNS IN NATURE – Summary Notes 1. ORGANISMS ARE MADE OF CELLS THAT HAVE SIMILAR STRUCTURAL CHARACTERISTICS 1.1 Outline the historical development of the cell theory‚ in particular‚ the contributions of Robert Hooke and Robert Brown The Cell Theory states that: All living things are made of cells (Theodor Schwann & Matthias Schleiden) Cells are the basic structural and functional unit of organisms (Theodor Schwann) All cells come from pre-existing cells (Rudolf Virchow) The historical
Free Cell Eukaryote
LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO.- 10800037 ROLL NO- RB1R07B02 B.TECH BIOTECH.(8th sem.)
Premium Bacteria Honey Agar plate
determine the antibacterial activity of Cannabis sativa leaf extract to some selective pathogenic bacterial strains such as Staphylococcus aureus‚ Escherichia coli‚ Pseudomonas aeruginosa. Antibacterial activity if cannabis sativa was evaluated by well diffusion method. The highest zone
Premium Antibiotic resistance Bacteria Penicillin
does magnetic field have on the formation of Liesegang rings (crystal growth in gel medium) on elements of ferromagnetic‚ diamagnetic and paramagnetic nature like Cobalt‚ Nickel‚ Copper and Manganese in their chloride compounds when performed in an agar medium? Lokesh Chandnani Candidate Number – 049369 – 0003 Supervisor: Mr. Jaydip Chaudhuri The Aga Khan Academy‚ Hyderabad Session: May 2015 Word Count: 3798 1. Abstract Liesegang rings phenomena is a special type of formation that occurs in
Premium Crystal structure Magnetism Magnetic field
All strains were provided by Ondokuz Mayıs University‚ the Faculty of Veterinary Medicine and Faculty of Medicine. Agar diffusion test The microbiological assays were performed under aseptic conditions inside a class 2 cabinet (Danlaf VFRS 1806‚ Denmark) previously sterilized. All testing procedures were carried out by a single operator using sterile instruments. Agar diffusion test (ADT) was used for determination of antimicrobial activities of test materials. The microorganisms inoculated in
Premium Bacteria Microbiology Water
Susceptibility test (disc diffusion technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile filter paper discs (using a disc dispenser)‚ which contain a known concentration of antibiotics. As soon as the antibiotic discs touch the agar‚ the antibiotic will begin to diffuse into the surrounding agar. During incubation the bacteria you inoculated onto the agar
Premium Bacteria Antibiotic resistance Microbiology
performed by agar disc diffusion method. Muella Hinton agar was prepared and autoclaved at a specified temperature and pressure. The medium was then cooled and 20mL of the medium was poured into a sterile petri dish and allowed to solidify. A sterile wire loop was used to transfer a colony of test organisms into a test tube with 2mL peptide water. A swab stick was dipped into the test tube containing the organisms and it was used to seed the organism on the solidified Muella Hinton agar. The prepared
Premium Bacteria Scientific method Escherichia coli
incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion is the movement of particles from an area of higher concentration to a lower concentration until they reach Equilibrium. (Hickman‚ 2008).Osmosis happens when water diffuses across the membrane from the region of lower solute concentration (higher
Premium Osmosis Cell membrane Red blood cell