90celcius which will convert the Chitin into Chitosan upon dissolving. The third phase will take place over 3 days. The nutrient broth and nutrient agar will be prepared first. On the 2nd day‚ the bacteria broth will be prepared prior to an overnight culture on the same day using E-Coli and M.Luteus. On the 3rd day‚ the Anti-bacterial well diffusion test will be carried out. Finally‚ we will
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sterilizing it with 70% ethanol. Then trypticase soy agar (TSA) was poured into six groups of 60 Petri dishes (See Appendix 1). The dishes were labeled based on the antibiotic used and were left to dry and solidify at room temperature. After an hour‚ the dishes were placed in a refrigerator at three degrees Celsius. If there was visible condensation on the agar plate‚ they were flipped to avoid the contact of heat that drove the moisture out of the agar dishes. The contents of the broth culture of E.
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in a color change of the medium from green to blue. Materials needed include simmons citrate agar slant tube‚ and a pure culture of bacteria. The starch hydrolysis test is used to determine the ability to hydrolyze starch. Bacteria use amylase‚ an extracellular enzyme to hydrolyze bonds linking the glucose subunits. Starch is detected by adding iodine to the medium. If the bacteria growing on the starch agar produce amylase‚ no color change will be seen around the bacterial growth because all of the
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identified the need to determine a given bacterium’s susceptibility or resistance to a given drug which prompted W. M. M. Kirby and A. W. Bauer to develop a single disk method for susceptibility testing. This experiment used the Kirby-Bauer disk diffusion test to measure the degree to which Penicillin‚ Streptomycin‚ Ampicillin‚ and Chloramphenicol inhibited the growth of the bacterium‚ Proteus vulgaris. The measured zone of resistance for each antibiotic was compared against antibiotic performance
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test antimicrobial activity against disease causing bacteria Staphylococcuaureus. Extracts of varying concentrations of Azadirachtaindica fruit pulp and leaf extract were prepared using Phosphate Buffer and tested against test organisms using agar diffusion method. Oxfloxacinof same varying concentrations were used to compare the effect of antimicrobial activity of fruit pulp and leaf extract. Keywords: Azadirachtaindica‚ Antimicrobial activity‚ Staphylococcus aureus. I. INTRODUCTION Azadirachtaindica
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Research II Summarized Project Proposal Title: The Efficacy of Lubi-lubi (Atropa belladonna) Fruit Extract as Anti-neoplastic Agent against Human Papilloma Virus Related Cervical Neoplasms Problem: Is the Lubi-lubi extract a potent anti-neoplastic agent against HPV related cervical neoplasms? Specific Problems: 1. What stage in the maturity of the deadly nightshade fruit is most efficient as an anti-neoplastic agent? 2. What concentration of the extract is most efficient
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points Describe the Kirby Bauer Test? Make sure you describe all the key elements. 1. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 2. Place antibiotic disks evenly spaced on the inoculated agar plates and incubate at 37C for 24-48 hours. 3. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 4. Using sterile technique place disk in each of the solutions:
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which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly with my results but Lysol was only 4mm more sustainable then Windex. Lysol
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INTRODUCTION Background Totipotency is the ability of a single cell to divide and produce all the differentiated cells in an organism‚ including extraembryonic tissues. Totipotent cells formed during sexual and asexual reproduction include spores and zygotes. Zygotes are the products of the fusion of two gametes. In some organisms‚ cells can dedifferentiate and regain totipotency. For example‚ a plant cutting or callus can be used to grow an entire plant. Human development begins when a sperm fertilizes
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group’s hypothesis was that “cells are small because small cells have a larger surface area to volume ratio”‚ which is important because a larger surface area allows for more molecules to diffuse at one time. The group created cells made out of blocks of agar-phenolphthalein. This allowed a simple indicator of pH‚ since phenolphthalein turns magenta when it comes into contact with a base.
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