potential. Methods Antibacterial activity of aqueous and organic seed extracts was assessed using agar diffusion assay‚ minimum inhibitory concentration and viable cell count studies; and their antibacterial effect was compared with some standard antibiotics. The presence of major phytoconstituents was detected qualitatively and quantitatively. The isolated phytoconstituents were subjected to disc diffusion assay to ascertain their antibacterial effect. Results Hot water and acetone seed extracts
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colloidal system consists of two separate phases: a dispersed phase (or internal phase) and a continuous phase (or dispersion medium). A colloidal system may be solid‚ liquid‚ or gaseous. Many familiar substances are colloids‚ as shown in the chart below. As well as these naturally occurring colloids‚ modern chemical process industries utilize high shear mixing technology to create novel colloids. The dispersed-phase particles have a diameter of between approximately 5 and 200 nanometers.[2] Such
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this carbon and it is a key enzyme in the bio refinery process of producing green chemicals (Ponnambalam et al.‚ 2011). In screening for the cellulolytic fungi‚ several qualitative display of cellulolytic such congo red clearing zone assay‚ gel diffusion assay and dyed congo red filter paper clearing zone assay can be used. In this screening‚ congo red clearing zone assay is performed on 9 unknown fungi isolates on CMC media. Cellulose degradation and its subsequent utilizations are important for
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bacterial cell wall‚ after this process has happened the antiseptic can stop the mutation of the bacteria and inhibit the growth of the bacteria or kill the bacteria. As you can see from my results the higher amount of antiseptic agent present in the ajar jelly‚ the more powerful it will be at destroying the bacterial cell wall‚ that is why with the increasing concentration of antiseptic the zone of inhibition increases because the high dose of concentration is attacking the bacteria‚ hence inhibiting a larger
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investigated compounds were dissolved in DMSO at 10 and 20 mg ml-1‚separately [19‚21‚25‚27‚34]. Nutrient agar was prepared‚ then sterilized in an autoclave and poured in sterile Petri plates. After cooling of nutrient ager in Petri plates‚ the studied organisms were grown on agar. After that‚ the sterili Paper discs of Whatman saturated with solution of investigated compounds were placed in the agar by working holes using a sterile crook borer. These Petri dishes were incubated for 24 h at 37oC [25]
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Banana and Papaya Fruit Peelings Extract as an Alternative Culture Medium for Fungi Submitted by: Maricel P. Clores Catherine A. Bernardo Rodora Lafiguera Julius Jorge Suarez Scope and Delimitation This study is focused on how to produce ointment out of banana and papaya fruit peelings extract to help avoiding fungal infections. It must be done within a laboratory. The place must be conducive for only a matter of years. This study builds upon on how to lessen fungal
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5 McFarland standard solution; a sterile cotton swab was dipped into the suspension‚ rotated several times‚ and pressed firmly on the inside wall of the tube above the fluid level removing excess inoculum. The surface of the agar plate was streaked over the entire sterile agar surface rotating the plate to ensure an even distribution of inoculum with a final swab around the rim. The plates need to dry for 3-5 min. Fifty uL aliquots of each test extract are dispensed into each well after the inoculation
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of bacteria in a given cultured sample is too great to be counted directly. The samples will be serially diluted by a factor of 10 in sterile saline‚ to be repeated 7 times. A 10 microliter aliquot from each dilution will be placed on a 5% blood agar plate or a MacConkey plate‚ depending on the type of organisms plated. These plates are selected because of the ease of differentiation between organisms. The original vial and all diluted aliquots will then be refrigerated to restrict growth until
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SFKL. S ECRETARIAT OF T H E P ACIFIC C O M M U N I T Y A Guide to the Common Edible and Medicinal Sea Plants of the Pacific Islands By Dr Irene Novaczek C ommunity Fisheries Training Pacific Series 3A S upplementary Guide to Sea Plants: Pacific Series 3 USP Marine Studies Programme / SPC Coastal Fisheries Programme: Training Materials for Pacific Community Fisheries The University of the South Pacific Secretariat of the Pacific Community Canada-South Pacific Ocean Development
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suface Smaller than most brown algae Rarely more than 3 or 4 ft. long Red in color‚ due to phycoerythrin -a red pigment present with chlorophyll The cell walls of Red algae: >have cellulose as a framework but are mostly containing mucilages contains agars and carrageenans‚ both of which are polysaccharide. Sold as food many thickeners. >Corallinered algae that form layers of calcium carbonate in their cell walls. >Phycobilins and caratenoids gives many red algae. Eg. Rhodymenia pseudopalmata- red or
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