resistance: intrinsic resistance and acquired resistance. There are some methods of observing the capability of the condiments in inhibiting the growth of microorganisms. In the experiment‚ the method used is well diffusion method‚ which used different type of condiments and added into holes of agar‚ where by the inhibition zone by the condiments could be observed. The larger the inhibition zone‚ the more effective the condiment was in inhibiting the growth of microorganisms. 1.2 Objectives By conducting
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THE EFFICACY OF TAKIP-KOHOL (Centella asiatica) LEAVES EXTRACT AS ANTIBACTERIAL AGENT AGAINST Escherichia coli‚ Salmonella enteritidis AND Staphylococcus aureus CLUSTER 1 LIFE SCIENCE TEAM CATEGORY 1|Page28 ABSTRACT THE EFFICACY OF TAKIP-KOHOL AGAINST (Centella Escherichia asiatica) coli‚ LEAVES Salmonella EXTRACT enteritidis AS AND ANTIBACTERIAL Staphylococcus aureus AGENT This research study aims to discover the efficacy of Takip-kohol leaves extract as antibacterial agent against Escherichia
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Microbiocidal Effects of Pure and Ethanolic Helianthus annuus extract University of Baguio Science High School Jasper Lorenz C. Choy February 2012 ABSTRACT Sunflowers in the Northern province of the Philippines were used by folks in order to treat injuries and for wound healing. Medically‚ sunflowers have not been fully analyzed for their active components. Literature states sunflower have vitamin E which are essential for human beings. Since the sunflower is an indigenous plant
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Harley−Prescott: Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on
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Bacterial colonies that have a different shape‚ size‚ or color are generally different bacteria. In this exercise you are streaking only one bacterium‚ so all the colonies should look pretty much the same. You will need the following: 2 tryptic soy agar (TSA) plates Broth culture of Escherichia coli After you have streaked your plates‚ they will be incubated at 37°C. The plates MUST be labeled and incubated in an inverted position. Next
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100 674.2 1076 29.5 TABLE 2: Antibacterial activity of different extracts of Annona squamosa by agar well diffusion method S.No. Doses of extract % Inhibition Zone of A. hydrophila(mm) Inhibition Zone of standard (mm) 1 25 No clear zone seen 8.1±0.36 2 50 No clear zone seen 9.16±0.57 3 75 No clear zone seen 10.33±0.47 4 100 16.5 ± 0.91mm 11.67±1.5 In
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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by analyzing deduced amino acid sequences of genes. SDS-PAGE analysis was carried out to estimate the molecular weights of proteins. Molecular weights of peh‚ celB and celC products were found to be 41.5 kDa‚ 29.5 kDa and 40 kDa‚ respectively. Agar diffusion assays were performed to qualitatively determine the activities of polygalacturonase and cellulase of the cloned
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The Antibacterial Effect of Papaya (Carica) Leaves Extract on Staphylococcus epidermidis Virgen Milagrosa University Foundation Special Science High School Martin P. Posadas Avenue‚ San Carlos City‚ Pangasinan Researcher S.Y. 2010-2011 The Antibacterial Effect of Papaya (Carica) Leaves Extract on Staphylococcus epidermidis A thesis Presented to the Faculty and staff of the Virgen Milagrosa University Foundation Special Science High School M.P. Posadas Ave.‚ San Carlos City
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