Agar well diffusion method was used to assess the activity against the bacteria by measuring zone of inhibition. Inhibiting concentrations used for all the extracts was 500 mg/ml. Methanolic extract of C. album was found to be most active among all other extracts
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| GRAM-POSITIVE BACTERIA * Alpha and gamma hemolysis on blood agar * Bacillus cereus colonies on blood agar * Beta-hemolysis on blood agar (S.aureus) * Beta-hemolysis on blood agar * Beta-hemolytic colonies on blood agar * Clostridium perfringens Gram stain * Corynebacterium diphtheriae Gram stain * Corynebacterium pseudotuberculosis on blood agar * Enterococcus faecalis * Enterococcus faecalis on blood agar * Enterococcus faecalis Gram stain * Enterococcus faecalis SEM
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INTRODUCTION The name agar originated from the Malay word “Agar-agar”‚ For the sake of simplicity agar-agar was shortened to just agar and is now accepted universally whether in the food and other industries. The use of agar became widespread in Indonesia‚ Malaysia‚ China‚ and Korea‚ where people called it Agar-Agar‚ which means seaweed. Its introduction to Europe dates from 1859‚ almost two hundred years later. Agar has been used in food preparations for over three hundred years. Agar-agar is also practiced
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Research Plan Title: In vitro Antibacterial Activity of Santol (Sandoricum koet jape) at Different pH of Agar School : Ramon Magsaysay (Cubao) High School School Address: Ermin Garcia St.‚ Cubao Quezon City Research Adviser: Mr. Ryan D. Balandra Statement of the Problem The aim of the study is to identify the effect of different pH level of the Agar plate to the antibacterial activity of Santol (Sandoricum koet jape). Specifically‚ the study will seek for the answer of the question:
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plates. This indicates that the Tn10 exposure was the cause of mutant variation. There were ten hypothesized auxotrophic colonies based on the comparison of mutagenized colonies in minimal and nutrient agar. These ten colonies were confirmed to be auxotrophic by their lack of growth in the minimal agar replica plate. The required growth factors for each auxotroph were determined based on which pool plates fostered growth for each streak. Auxotrophs 1‚ 2‚ 3‚ and 4 require valine as a growth factor
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of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth. (1) 2(A). Experiment 1: Choose 2 words that describe the appearance of the pre –incubation broth: (i) Straw yellow
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The repulsion and fear have originated from the fact that these creatures can be quite beautiful to behold‚ but extremely painful when stung by them. These marine creatures are classified under the group of Cnidarians. They typically have a soft jelly like consistency to their body‚ prompting the people to call them as Jellyfish. There are numerous surprises in store‚ when we deal with Jellyfishes. The first and foremost of the surprise is the vast reservoir of chemical factories‚ inside the tiny
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organisms can easily be seen using differing types of agar‚ which creates an ideal environment for the organisms to form colonies‚ which are groups of hundreds of organisms that can be seen with the naked eye. In order to see individual microorganisms‚ it is necessary to use the magnification of a high-powered microscope. These techniques of microbiology are used in the following five experiments. The first experiment used Trypticase Soy Nutrient Agar (TSA)‚ which can grow a wide variety of organisms
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for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars‚ various other tests need to be done to differentiate and confirm their identity. RESULTS Bacteriology – When grown on MacConkey agar‚ A had abundant growth of pink punctiform colonies that are circular and moist. Microbes A are gram-negative cocci. B had moderate growth of yellow punctiform colonies that are circular
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David Kennedy Bio 210 Lab Report 1 10/11/13 Microbial Growth Background Information: This lab was conducted in order to understand basic differences among differential and selective media‚ while recognizing how each media is used to isolate and identify microorganisms (Wistreich‚ 2003). The first microorganism analyzed was Staphylococcus epidermidis. This organism is gram-positive‚ single celled‚ arranged in grape-like clusters‚ and cocci in shape (Bukhari‚ 2004). S. epidermidis is approximately
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