The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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In each tube a different kind of unknown bacteria was growing on the agar slant. Having the slanted agar allows for more surface area‚ therefore‚ more room for bacteria to grow. For each test there is a general set of rules to follow when transferring bacteria from one culture to another. Disinfect the table surface. Sterilize the transfer
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(in different amounts)‚ which can speed up the breakdown of proteins. As milk-agar plate is a milk protein‚ so when it is incubated with fruit juices containing proteases‚ the milk protein will be broken down and clear zones will appear around the wells containing different fruit juices. Thus‚ the higher the protease activity‚ the larger the diameter of the clear zones. Equation: Control: One well in the milk-agar plate is filled with distilled water to act as a control to show that the formation
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3 antibiotic dicks | Distilled water | Culture of Serratia marcesans | Sterile filter-paper disks | Transparent tape | 1 sterile nutrient agar plate | Metric ruler | Forceps | 1 sterile cotton swab | Safety goggles | Lab apron | | Materials: Procedure: In the Antibiotic Resistant Bacteria lab‚ the effect of different antibiotics on the zone of inhibition on bacteria‚ Serratia marcesans‚ was measured in millimeters. The safety equipment‚ lab apron and goggles were worn at all times
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Some food in this world are made using microorganism to produce a desire flavour‚ taste and texture of the food. For examples: yogurt‚ tapai‚ cheese‚ bread and others. Starter cultures is used in these food production. A starter culture is a microbiological culture which actually perform fermentation. These starters usually consist of a cultivation medium‚ such as grains‚ seeds‚ or nutrient liquids that have been well colonized by the microorganisms used for the fermentation. These starters are formed
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Osmosis and the Egg Lab Report By: N. Mullins Date: January 20‚ 2011 Introduction This lab was designed to explain the different things osmosis does. Osmosis is the diffusion of water across a semi permeable membrane. Over three days we tested the egg in three different solutions to observe the changes. On Day 1‚ the main question we answered was what happened to the egg when it was placed in vinegar? Before doing this I came to my hypothesis was that the vinegar would eat away at the shell and eventually
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designed Rainbow Agar O157 for the isolation and identification of EHEC. In this medium E. coli O157 was characterised by black colonies whereas O113 and some other EHEC strains were mauve‚ red or pink and indistinguishable from other strains of E. coli. Manafi and Kremsmaier (2001) evaluated the efficiency of different media for detecting Escherichia coli O157:H7. They found that SMAC agar had a sensitivity and specificity of 94.1 per cent and 91.6 per cent‚ Fluorocult HC agar
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correct‚ I carried out an experiment where I grew bacteria on 14 different agar plates and placed garlic on seven of the agar plates and then had no garlic present in the other seven agar plates. All 14 agar plates were kept under the same conditions while the bacteria colony was growing in order to ensure that all factors were kept constant. From the results‚ it was evidently clear that the size of the bacteria colony of the agar plates without garlic with an average of 115.29 mm² was much greater than
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Prevalence of clarithromycin resistance in H.pylori patients with failure of treatment Introduction; The H.pylori is a gram negative bacterium that colonizes the stomach has a high prevalence; more than 50 percent of the global population has H.pylori. It’s influenced by geography‚ age‚ gender‚ socioeconomic status‚ although it is decreasing in the developed world‚ it remains high in the developing world as there is less proper sanitation and hygiene‚ crowdedness‚ lack of safe water supply
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causative bacteria or fungus. Potato dextrose agar is a good nutrient agar for mycelia to thrive on which is present in most fungal moulds.1 Standard nutrient agar is a general utility used for non-fastidious microorganisms.2 Aim The aim is to isolate fungi and bacteria colonies from diseased and healthy leaves. Materials and Methods Materials used for the experiment was two of each: standard nutrient agar plate and potato dextrose agar plate. To remove any epiphytic or saprophytic
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