Materials: 4 sterile cotton swab‚ sterile water in test tube‚ 4 agar plates and 2 blood agar plates‚ wax pencil and labels. Procedure: Appropriately label the cover of each plate as indicated in lab. 1. Determine 2 sampling sources‚ one from your body and one from the surrounding environment. 2. For the first agar plate‚ for sampling from air‚ remove the lids from the plate and allow it to sit uncovered for 15 minutes. 1. For second agar plate‚ open the “stick” end of the sterile cotton swabs to
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observable microbial colonies on the surface of the Agar solid‚ as to determine the presence of microbes in consumable products i.e. yoghurt and blue vein cheese. HYPOTHESIS: Microbial growth will be present in two of the three Agar plates (those containing the food product) due to the suspected presence of microbes‚ whilst the control Agar plate (containing no food products) will remain free of contamination and microbial growth. MATERIALS: - 3x Agar plates - 10g of berry yoghurt - 10g
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specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain‚ Catalase‚ Mannitol Salt Agar (MSA)‚ Blood Agar‚ Novobiocin‚ Coagulase‚ and DNAse (Alachi‚ 2007). Rebekah Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the
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such as the mouth‚ blood vessels‚ heart and lungs and make up the outer layers of the skin. The bottom layer of the cells is attached to the basement membrane for support and connection. The main function is allowing materials to pass through via diffusion and osmosis. Cuboidal Epithelium. Cuboidal cells are roughly‚ a cuboidal in shape. Each cell has a spherical nucleus in the centre. Cuboidal epithelium is found in glands and in the lining of the kidney tubules as well as in the ducts of the
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was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were identified as Staphylococcus aureus with a positive confirmatory test‚ mannitol salt agar‚ showing consistent results as well for S. aureus. The Gram-negative bacteria were Pseudomonas aeruginosa with a positive confirmatory
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distending the cell Bacillus cereus Table 2. The observation of the colonies on the nutrient agar plates after incubated Three isolation techniques were used; streak plate‚ spread plate and pour plate and the agar plates were then inverted and incubated at 370C for one day. The distributions of colonies were then observed. Observations were recorded. Isolation Techniques Observation on the nutrient agar plates Streak plate At the first inoculum‚ all the bacterial colonies were overlapping with each
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of a wide variety of microorganisms whilst the agar function is the solid media onto which the bacteria can be isolated as independent colonies which are representatives of different bacterial species. Controlling microbial growth is necessary in numerous situations and is greatly significant in areas such as medicine. Growth of microorganisms is
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Abstract Microorganisms are plentiful and widespread in the environment. In this lab‚ we undertook to determine the differences in the agars being used and the different colony count observed. After taking four different samples of microbes from the environment and swabbed them in two different plates one with nutrient agar and the other with sabouaud dextrose agar. After the microbes had incubated for 48 hours no results were discovered from the swabs we had taken from the environment. This lab
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dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod‚ culture was taken from the tube and dragged across area 1 several time‚of the agar. The agar was then turned 90º‚ and the loop was flamed and cooled. Taking some culture from area 1‚ it was dragged over area two‚and the same steps were done for areas 3 and 4.Another technique used was spread-plate‚ where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod. The rod
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or magnesium environment will be significantly different than the rate of streaming in plain 2% agar and that calcium and magnesium will have an equal effect on cytoplasmic streaming in Physarum polycephalum because of their similar chemical properties. Methods: To set up P. polycephalum samples‚ 15 plates of agar were set up to culture the mold. After mixing 2M solutions of calcium and magnesium‚ agar was measured and mixed into the solutions and 5 plates of calcium solution‚ 5 plates of magnesium
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