controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar upon feeding bacteria to it. 2. Why shoudn’t a student swab his or her mouth or cough onto an agar plate to initiate a culture? Even though most bacteria in the human body is harmless‚ if one was knowingly or unknowingly ill then harmful
Premium Bacteria Petri dish Agar plate
bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface of Simmon’s citrate agar. Followed by overnight incubation at 37°C. Day after the slant
Premium Escherichia coli Escherichia coli PH indicator
_____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform pore size than that of agar. It is also easily poured and does not denature the samples. In agarose gel electrophoresis‚ DNA or RNA fragments are separated or isolated according
Premium DNA Molecular biology Gel electrophoresis
plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate and isolate this microorganism from the many other microorganisms with which
Premium Bacteria Microbiology Agar plate
A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot
Free Agar plate Petri dish Fungus
ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and
Free Bacteria Agar plate
Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
Premium Bacteria Microbiology Agar plate
(positive). The catalase test result indicated that my unknown was negative because no bubbles formed when I placed a loop of the organism into hydrogen peroxide. The next step was to examine a blood agar plate to examine the colonial morphology of hemolysis ‚ my organism produced gamma hemolysis on the blood agar because there was no hemolysis on the plate after 48 hours incubation period. After examining my unknown for hemolysis I set a series of five experiments: Bacitracin SX CAMP Enterococcosel
Premium Bacteria Streptococcus Agar plate
water because without anything to help it‚ water cannot do anything. For example‚ if you wash your hands and you do not use any soap‚ none of the germs on your hand will be cleaned. Materials: The materials we used in this lab were a petri dish with agar‚ forceps‚ water‚ alcohol‚ hydrogen peroxide‚ antibacterial soap‚ filter paper disc‚ tape‚ and a permanent marker. Procedure: We started this lab on May 12‚ 2014. Using a permanent marker on the bottom of the dish‚ we divided it into 4 sections.
Premium Oxygen Bacteria Agar plate
MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the
Premium Gram staining Staining Escherichia coli