Abstract Introduction An antimicrobial is a substance that kills or inhibits the growth of microorganisms such as bacteria‚ fungi‚ or protozoans (Antimicrobial). Antimicrobial drugs either kill microbes or prevent the growth of microbes. Disinfectants are antimicrobial substances used on non-living objects or outside the body. Ginger Figure 1 : Ginger (Studies Reveal Ginger Lowers Colon Cancer Risk) Ginger is commonly used around the world and has been employed in the treatment‚ cure‚ and
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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TSA (tryptic soy agar) media was placed in a different place to collect cells as well as printed with a thumb print from each member of the group. Incubation is used because each sample needed a certain temperature for the cells to grow. Inspection was used to look at each plate and see how many colonies are found. One TSA plate was placed wherever the student wanted in the building and the other was for each group member to press their thumb print in. The SBA (sheep blood agar) was used for a mouth
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MgSO4 .7H2O‚ 0.2; CaCl2‚ 0.55; NH4Cl‚ 0.4; agar‚ 1.5. A stock solution of the dye was prepared and used for all studies. The sample collected was screened for CV dye decolorizing bacterial strains by inoculating 10 ml of wastewater into flask containing 100 ml of MSM broth. The flasks were incubated at 35°C under shaking conditions (120 rpm). After 48 hr of incubation period‚ 1ml of the culture broth was appropriately diluted and plated on MSM-CV dye amended agar plates. The morphologically distinct bacterial
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cotton with the sterile water then rub gently over areas of the body assigned each group. 1 and 2 – mouth ‚ cheek‚ nose‚ ear‚ nape 3 and 4 – mouth‚ between toes and fingers‚ arm‚ throat 5 – mouth‚ ear‚ between fingers ‚ nose‚ arm 3. Swab the agar plates direct with the cotton swab by simple streaking. 4. Label the plates and incubate for 24 hours. 5. Examine the plates (note the colonies). Experiment #2Normal Flora of the Body | NA | Area Sampled | Color | Whole colony appearance
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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merely have greatly reduced numbers of “harmless” bacteria. It is often necessary to determine how many live bacteria are actually in a sample‚ especially when measuring growth rates or determining disinfectant effectiveness. This involves MacConkey agar which is a selective and differential
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unknown mixture‚ using a number of laboratory tests. Over the semester‚ multiple tests were carried out to identify between gram-positive and gram-negative bacteria including the Mannitol Salt Agar (MSA) media test‚ which selects only for gram positive bacteria‚ and the use of Eosin Methylene Blue Levine (EMB) Agar media‚ which selects for gram negative bacteria and differentiates between lactose fermenters (paracolons) and non-lactose fermenters (coliforms).These tests along with other selective and/or
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ATCC 8007. The bacterium was activated in Man-Rogosa-Sharpe (MRS) agar medium (Difco‚ BD Diagnostic Systems‚ Maryland‚ USA) consisting of (g L1): peptone casein‚ 30; meat extract‚ 10; yeast extract‚ 6.0; sodium acetate‚ 5.0; ammonium citrate‚ 2.0; glucose‚ 0.2; magnesium sulfate‚ 0.2; manganese sulfate‚ 0.05; dipotassium phosphate‚ 2.0. The pH of the medium was adjusted to 6.5. The master cell bank culture was inoculated into agar plates and incubated for 48 h to produce the working cell culture
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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