sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate. 5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate‚ making sure the lid of the culture container does not touch the surface‚ and that the lid of the agar plate is quickly replaced after this step has been carried out. 6. Sterilise the grabbing part and up the handle
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in a color change of the medium from green to blue. Materials needed include simmons citrate agar slant tube‚ and a pure culture of bacteria. The starch hydrolysis test is used to determine the ability to hydrolyze starch. Bacteria use amylase‚ an extracellular enzyme to hydrolyze bonds linking the glucose subunits. Starch is detected by adding iodine to the medium. If the bacteria growing on the starch agar produce amylase‚ no color change will be seen around the bacterial growth because all of the
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____________________ ABSTRACT The effect of molecular weight on the diffusion of substances were tested through the use of agar-water gel with three wells placed in a petri dish. Drops of three different substances namely: Potassium permanganate (KMnO4)‚ Potassium dichromate (K2Cr2O7) and Methylene Blue were placed in the wells of the agar-water gel in the petri dish. The diameter of the diffused substance was noted every 3 minutes for 30 minutes. The Potassium permanganate (KMnO4)
Free Diffusion Molecular diffusion Ammonia
INTRODUCTION Background Totipotency is the ability of a single cell to divide and produce all the differentiated cells in an organism‚ including extraembryonic tissues. Totipotent cells formed during sexual and asexual reproduction include spores and zygotes. Zygotes are the products of the fusion of two gametes. In some organisms‚ cells can dedifferentiate and regain totipotency. For example‚ a plant cutting or callus can be used to grow an entire plant. Human development begins when a sperm fertilizes
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investigation of the effect of fresh pineapple on the setting of jelly Propose a hypothesis (p. 1-2) It is the fresh pineapple that causes the jelly to remain in liquid form. Design and perform an experiment (p. 1-2) 1 (Answer varies with Ss. The recommended quantity of jelly powder and water is stated on the packet of the jelly powder. Jelly will not set if it is too dilute.) 2 Method II. This makes sure the concentrations of the jelly solutions in different containers are the same. A Identifying
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colloidal system consists of two separate phases: a dispersed phase (or internal phase) and a continuous phase (or dispersion medium). A colloidal system may be solid‚ liquid‚ or gaseous. Many familiar substances are colloids‚ as shown in the chart below. As well as these naturally occurring colloids‚ modern chemical process industries utilize high shear mixing technology to create novel colloids. The dispersed-phase particles have a diameter of between approximately 5 and 200 nanometers.[2] Such
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bacterial cell wall‚ after this process has happened the antiseptic can stop the mutation of the bacteria and inhibit the growth of the bacteria or kill the bacteria. As you can see from my results the higher amount of antiseptic agent present in the ajar jelly‚ the more powerful it will be at destroying the bacterial cell wall‚ that is why with the increasing concentration of antiseptic the zone of inhibition increases because the high dose of concentration is attacking the bacteria‚ hence inhibiting a larger
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5 McFarland standard solution; a sterile cotton swab was dipped into the suspension‚ rotated several times‚ and pressed firmly on the inside wall of the tube above the fluid level removing excess inoculum. The surface of the agar plate was streaked over the entire sterile agar surface rotating the plate to ensure an even distribution of inoculum with a final swab around the rim. The plates need to dry for 3-5 min. Fifty uL aliquots of each test extract are dispensed into each well after the inoculation
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SFKL. S ECRETARIAT OF T H E P ACIFIC C O M M U N I T Y A Guide to the Common Edible and Medicinal Sea Plants of the Pacific Islands By Dr Irene Novaczek C ommunity Fisheries Training Pacific Series 3A S upplementary Guide to Sea Plants: Pacific Series 3 USP Marine Studies Programme / SPC Coastal Fisheries Programme: Training Materials for Pacific Community Fisheries The University of the South Pacific Secretariat of the Pacific Community Canada-South Pacific Ocean Development
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suface Smaller than most brown algae Rarely more than 3 or 4 ft. long Red in color‚ due to phycoerythrin -a red pigment present with chlorophyll The cell walls of Red algae: >have cellulose as a framework but are mostly containing mucilages contains agars and carrageenans‚ both of which are polysaccharide. Sold as food many thickeners. >Corallinered algae that form layers of calcium carbonate in their cell walls. >Phycobilins and caratenoids gives many red algae. Eg. Rhodymenia pseudopalmata- red or
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