Project 1: Antimicrobial Activity of Soil Isolates John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining
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specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain‚ Catalase‚ Mannitol Salt Agar (MSA)‚ Blood Agar‚ Novobiocin‚ Coagulase‚ and DNAse (Alachi‚ 2007). Rebekah Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the
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was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were identified as Staphylococcus aureus with a positive confirmatory test‚ mannitol salt agar‚ showing consistent results as well for S. aureus. The Gram-negative bacteria were Pseudomonas aeruginosa with a positive confirmatory
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distending the cell Bacillus cereus Table 2. The observation of the colonies on the nutrient agar plates after incubated Three isolation techniques were used; streak plate‚ spread plate and pour plate and the agar plates were then inverted and incubated at 370C for one day. The distributions of colonies were then observed. Observations were recorded. Isolation Techniques Observation on the nutrient agar plates Streak plate At the first inoculum‚ all the bacterial colonies were overlapping with each
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Abstract Microorganisms are plentiful and widespread in the environment. In this lab‚ we undertook to determine the differences in the agars being used and the different colony count observed. After taking four different samples of microbes from the environment and swabbed them in two different plates one with nutrient agar and the other with sabouaud dextrose agar. After the microbes had incubated for 48 hours no results were discovered from the swabs we had taken from the environment. This lab
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dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod‚ culture was taken from the tube and dragged across area 1 several time‚of the agar. The agar was then turned 90º‚ and the loop was flamed and cooled. Taking some culture from area 1‚ it was dragged over area two‚and the same steps were done for areas 3 and 4.Another technique used was spread-plate‚ where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod. The rod
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or magnesium environment will be significantly different than the rate of streaming in plain 2% agar and that calcium and magnesium will have an equal effect on cytoplasmic streaming in Physarum polycephalum because of their similar chemical properties. Methods: To set up P. polycephalum samples‚ 15 plates of agar were set up to culture the mold. After mixing 2M solutions of calcium and magnesium‚ agar was measured and mixed into the solutions and 5 plates of calcium solution‚ 5 plates of magnesium
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area- L x W x 6 and compare it with the volumes. The formula to determine volumes of cubes is L x W x H. Although that type of experiment will show no insight into SA/V ratio in relation to the rate of diffusion. Equipment 1. Agar-phenolphthalein - sodium hydroxide jelly 2. O.1 M hydrochloric acid 3. Ruler (cm and mm) 4. Razor blade 5. Paper towel 6. Beaker Method 1. A block of gelatin which has been dyed
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distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the smear was sequence covered with crystal violet
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SUMMARY PAGE CONTENTS PAGE INTRODUCTION As part of a balanced diet‚ most individuals incorporate wheat products into their diets; bread‚ cakes‚ pizzas or biscuits are just the few gluten containing products that people without coleiac disease‚ take for granted. Although deemed as normal for most‚ these simple foods are not as nutritious or beneficial to people who have Coeliac disease. Coeliac disease pronounced (see-liac) is intolerance to wheat products‚ whereby gluten fights against
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