(Pearson’s 2011). Mannitol Salt Agar The Mannitol Salt Agar test was used to test only the Unknown Gram-positive bacteria. One colony from the Unknown 13A plate was used to inoculate the Mannitol Salt Agar Plate. After the plate was inoculated‚ it was placed in an incubator at 35ºC for 48 hours. If the agar surrounding the culture changed from red to yellow the test was positive‚ which indicated that the bacterium was capable of fermenting mannitol (Pearson’s 2011). If the agar surrounding the culture did
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ABSTRACT Nature has been a source of medicinal agents for thousands of years and an impressive number of modern drugs have been isolated from natural sources Plants used for traditional medicine contain a wide range of substances that can be used to treat chronic as well as infectious diseases. Clinical microbiologists have great interest in screening of plants for antimicrobial activities and phytochemicals as potential new therapeutics. The use of plant extract for medical treatments is enjoying
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pigmentation‚ colony‚ margin characteristics‚ elevation properties‚ broth characteristics and agar stroke properties. 2. To examine bacteria growth characteristics on different culture media. Introduction: Bacterial species can sometimes be identified on the basis of how they appear on or in the different media. The pigmentation‚ size and shape of bacterial colonies as they grow on and in agar plates can provide identifying signs. Observing the growth characteristics of organisms in broth
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Case Study Report for Unknown #49 A new school for girls was opened in a larger village in Northern Pakistan‚ and the teachers were proud to have convinced many families in the surrounding villages to allow their daughters a basic education. The school was a success‚ and the 6-10 year olds girls quickly learned how to read and write and were even instructed in the sciences. However‚ 2 weeks before summer break‚ many of the girls and the teacher developed breathing problems that included severe coughing
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Abstract In an environment isolation procedure‚ experiments under categories‚ such as‚ morphology‚ physiology‚ antibacterial susceptibility‚ selective media‚ and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to
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be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen. Hypothesis: For this experiment‚ antibodies will be placed in wells on an agar plate‚ surrounded by wells containing serum (proteins‚ antigens etc.). If the antibody interacts with the antigen of surrounding wells‚ then the presence
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diffusion Materials: Agar‚ Ruler Chemicals: Potassium Permanganate‚ Methylene Blue and Potassium Dichromate. Method: Obtain a pinch of potassium permanganate‚ methylene blue and potassium dichromate. Place each substance carefully on the surface of agar‚ carefully noting which is which so that the crystals are spread equally over the agar and not too close to the edge of the dish. Measure the diameter of the colored area immediately after adding the substance to the agar with regular 10-minute
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for their industrial production are Bacillus subtilis‚ Bacillus licheniformis‚ Bacillus amyloliquifaciens and Aspergillus niger General Lab Requirements: • Autoclave or pressure cooker • Hot Plate or Microwave oven • Nutrient Agar powder • Potato Dextrose Agar powder • Soluble starch • Weighing scales • Shaker • Spectrophotometer or colorimeter • Water bath (Temperature controlled) Materials per group of 4 students • Hand trowel or disposable spoons • Sterile pipettes (One each of
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of LB agar. We then put 250 µn of CaCl2 transformation solution into two micro test tubes for the purpose of changing the bacterium’s cell wall to allow the pGLO plasmid to enter more easily. Second‚ we placed one colony of E. Coli from our original plate into each of the micro test tubes. Subsequently‚ we extracted a loopful of pGLO plasmid with a sterile loop and then placed it in one of the micro test tubes. We incubated the tubes on the ice for ten minutes while we prepared four new agar plates
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guess at what is causing the infection and what antibiotic is needed so that treatment can start right away. • Necessary to test which antibiotics the microorganism is sensitive to. • Place discs soaked in different antibiotics onto agar jelly on which the bacterium is growing. • If a clear area of a certain diameter appears around the disc the antibiotic is effective. [pic] [pic] Antibiotic Resistant Bacteria Are: Bacteria mutate and are able to resist the antibiotics
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