ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY‚ 2013.SUPERVISOR- PROFESSOR N. SMITH | | Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
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Brain break- what type of medium is blood agar- COMPLEX Differential vs selective vs differential AND selective media Differential- ingredient added that can be changed by certain bacteria in a recognizable way Blood agar Hemolysis Alpha- green Beta- yellow surrounding strep throat Selective Hemolytic Ingredient added that inhibits growth f many unwanted organisms Antibiotic-containing medium Selective and differential MacConkey agar – gram neg that ferment lactose Bile
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Example − Sargassum‚ Laminaria‚ and Porphyra used as food * Some brown and red algae species produce water-holding hydrocolloids. Example − Algin (brown algae) and carrageen (red algae) * Agar produced by Gelidium and Gracilaria is used to grow microbes and in preparation of ice creams and jellies. * Chlorella and Spirulina are protein-rich unicellular algae‚ used as food supplements. They are also known as space food. *
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experiment. In part A of the lab‚ diffusion was demonstrated with two solids and an agar gel petri dish. One crystal of potassium permanganate and one crystal of methylene blue were placed on either side of an agar gel petri dish. The purpose of this experiment was to determine which of the crystals would diffuse across the gel more. So the question is‚ which solid would have a higher rate of diffusion through the agar‚ methylene blue or potassium permanganate? Diffusion is the movement of particles
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antibiotic-producing microbes in the soil that was taken from a local sample. To do so‚ two different samples of bacteria have been taken and placed onto agar plates (labelled A and B). As we allow each sample to grow‚ our objective will be to see what‚ if any‚ antibiotics develop and inhibit the growth of the bacteria. We predict that antibiotics will form on the agar plates‚ thus inhibiting the growth
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of diffusion in agar means it will diffuse faster because it is more concentrated. We graphed the diffusion of chloride ions rather than sodium ions because while the sodium ions do diffuse in to the agar the chloride ions from the NaCl will combine with silver ions‚ from AgNO3 and create a white precipitate. The white precipitate allows us to measure the the white band through the agar at different points and determine the rate of diffusion of the chloride ions. If the the agar concentration was
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Background: In Jane Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus
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Absract The aim of the prac was to identify and isolate Staphylococcus aureus and Eschericia coli in milk and salmonella in poultry.It was to investigate bacteriological quality of milk and poultry. Salmonella is areprobably the most important cause of food borne illness globally.Staphylocooccus aureus all cause food borne diseases if consumed in a contaminated milk. For milk a spread plate method was used and for poultry a streak method was applied using different Medias. All food contain a certain
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aureus. Introduction: The purpose of this lab was to determine the identity of an unknown bacteria slant culture using a series of differential tests. The tests used to identify the unknown bacterial culture included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows for the fast and easy detection between gram negative and
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Selective enrichment broth- with Tetrathionate Brilliant Broth Selective Plating- Brilliant Green Agar Isolation of salmonella conformation- is preformed using TSI and LIA Test results Tetrathionate Brilliant Green Broth (TGBG)was inoculated from the whirl pack spinach infected bag. TBGB inhibits the growth of organisms other than salmonella. The next test preformed was the Brilliant Green Agar (BGA)
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