The first experiment involved agar cubes‚ each with a different surface area‚ to measure how surface area affects the rate of diffusion. In this experiment‚ the constants included 2% agar containing the pH indicator dye phenolphthalein‚ 1% phenolphthalein solution‚ 0.1M NaOH‚ 0.1M HCl‚ squares of hard‚ thin plastic (from disposable plates); unserrated knives; or scalpels from dissection kits‚ metric rulers‚ petri dishes or test tubes to hold the agar cubes. For the independent variable‚
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discovered by Lederberg and Tatum in 1946. In their experiment‚ they grew two strains of bacteria in separate vessels with rich medium and then together in one vessel containing the same medium. Then‚ they spread the three vessel contents onto medium agar plates and incubated them overnight at 37˚C. The only plate that showed cell growth was the plate containing the mixture of the two bacterial strains. The other two plates showed no growth. This experiment proved that in order for recombination to
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For experiments 1and 2‚ many different tests were conducted in order to investigate the different microbes found on the different types of turkey sampled. To begin‚ our group obtained 5 different forms of turkey that are encountered in everyday life: Inspirations deli turkey from Hannaford supermarkets‚ turkey bacon‚ turkey jerky from Whole Foods‚ Inspirations ground turkey from Hannaford‚ and a raw whole chunk slice of turkey breast form Whole Foods‚ these foods were considered experiment 1. Experiment
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Historical synonym: Bacillus difficile. Morphology Gram-positive‚ straight rods‚ motility is variable‚ have a flagella. Spores are oval‚ sub terminal swelling the cell. Cultural characteristics Colonies on blood agar plates are circular‚ flat or low convex‚ opaque‚
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Title: Screening of Cellulolytic Activity of Locally Isolated Thermophilic Fungi Title : Screening of Cellulolytic Activity of Locally Isolated Thermophilic Fungi Objective : To screen for thermophilic fungi as producer of fungal cellulase. Introduction One of the most important sources of carbon that is abundantly found on this planet is cellulose. While cellulase is the enzyme to degrade this carbon and it is a key enzyme in the bio refinery process of producing green chemicals
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culture on the EMB-lactose and PEA. Vancomycin wasn’t available to be used. We added our live culture to the EMB-lactose and PEA and added sterile beads to spread the bacterial cells all over the surfaces of the two agars and removed the beads and incubated the two agars. While the agars were incubating‚ we prepared our gel and loaded our respectful samples and ran the gel. After the gel finished running‚ we got a picture of our gel and recorded our observation for later
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Microbiology Module 02 Homework Assignment Use the information presented in this module along with additional outside research to answer the questions: 1) Compare and contrast prokaryotic and eukaryotic. a) Prokaryotes and eukaryotes are two types of cells that are very different but share some certain properties such as methods of reproduction‚ protein synthesis‚ an organized metabolism‚ response to stimuli‚ and plasma membranes. One significant difference is that prokaryotes are without a cell
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results of my unknown and Salmonella typhimurium found in the Bergey’s manual. Gram staining showed gram negative rods‚ a motility test was also conducted to see if the bacterium moved or not‚ it was found to be none motile. Three different types of agar plates were used‚ they had two known bacterium put on along with the unknown to be able to compare negative and positive results if the known with the results of the unknown‚ refer to Barbaro (2016) for how the test were done. Table 1: Results of
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ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
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some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows: Lab day 1; today in lab we obtained the unknown mixed culture “041”and one brain-heart infusion agar (BHIA). The first step was the preparation of the medium‚ the bottom of the BHIA dish was labeled with the bacterium number‚ initials‚ and section; then divided into four quadrants. The second step‚ we used the septic technique to transfer a small
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