small end bent to close it. * 1 Sharpie marking pen. * 1 glass test tube with a cap containing 2 ml of sterile nutrient broth and labeled "Broth". * 2 Petri dishes containing only nutrient agar and labeled "No Amp" on the bottom with date. * 2 Petri dishes containing nutrient agar and the antibiotic ampicillin. The dishes should be labeled "Amp" on the bottom with the date. * The laboratory instructions. Methods: Pre-Lab: * PREPARATION OF THE E. COLI STARTER
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aureus were maintained on Tryptic Soy agar (TSA)‚ following incubation at 37 oC for 24 h. Inocula were obtained from overnight fresh cultures adjusted to approximately log 108 CFU/ml‚ a turbidity equivalent to a 0.5 McFarland standard (Kroning et al.‚ 2016). The inocula were spread on the surface of Mueller-Hinton agar (MHA) plates. The disk diffusion test‚ recommended by the Clinical and Laboratory Standards Institute (CLSI‚
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WUS101/2 TERAS KEUSAHAWANAN SEM II 2012/2013 LAPORAN PROFIL USAHAWAN Prepared by: NORHAMIZAH BINTI IDROS 108965 930623-10-7864 KEJURUTERAAN ELEKTRONIK (EE) Prepared for: MOHD KAMIL ARIFFIN LECTURER WUS101 USM 25 APRIL 2013 Date: 20 APRIL 2013 Norhamizah Idros Kampus Kejuruteraan Universiti Sains Malaysia 14300 Nibong Tebal‚ Pulau Pinang. Mohd Shafie Ariffin Lecturer of WUS101 Universiti Sains Malaysia. Submission of WUS101
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What Sunscreen really works? Problem: Will sunscreen with an SPF of 4 be able to withstand the sunlight more effectively than SPF 45? Purpose: Due to the increasing amount of sun exposure on teens‚ we want to ensure that sunscreen can protect against the sun. An increase amount of exposure can damage the skin directly. The dangers of increased sun can lead to the 3 types of skin cancer (Basal‚ Melanoma‚ and Squamous) and these types can spread to other parts of the body. The most dangerous form
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Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then the bacteria is non-hemolytic. For my bacteria no change is observed in the media therefore the bacteria is non-hemolytic and negative result. MacConkey Agar test
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of bacteria in a given cultured sample is too great to be counted directly. The samples will be serially diluted by a factor of 10 in sterile saline‚ to be repeated 7 times. A 10 microliter aliquot from each dilution will be placed on a 5% blood agar plate or a MacConkey plate‚ depending on the type of organisms plated. These plates are selected because of the ease of differentiation between organisms. The original vial and all diluted aliquots will then be refrigerated to restrict growth until
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technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile filter paper discs (using a disc dispenser)‚ which contain a known concentration of antibiotics. As soon as the antibiotic discs touch the agar‚ the antibiotic will begin to diffuse into the surrounding agar. During incubation the bacteria you inoculated onto the agar
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This is a quantitative test and is considered to be the gold-standard method for biofilm detection (13). Organisms isolated from fresh agar plates were inoculated in 10 mL of trypticase soy broth with 1% glucose. Broths were incubated at 370C for 24 hours. The cultures were then diluted 1:100 with fresh medium. Individual wells of sterile 96 well-flat bottom polystyrene tissue culture
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was clearing off the DVR and catching up on old episodes of The Martha Stewart Show‚ I spotted an episode description that said “Jelly Mongers”. I’ve been in a canning mood this summer‚ so I figured this would be something informative for me to watch. Well‚ it turns out that it WAS very informative‚ but it also turned out to NOT be about canned jelly. Of course the “jelly” referred to was Jell-O basically. I know from my studies of historic culinaria (and endlessly watching British movies and TV shows)
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Pharmaceutical Microbiology CLASS CODE: MP202 SARA ASHOUR Signature ……………………………….. Date ……………………………….. Total Word Count 1492 I declare that‚ except where specifically indicated‚ all the work presented in this report is my own and I am the sole author of all parts. Toxic shock syndrome (TSS) is an uncommon but serious systemic infection which can occur in males and females of all ages. Symptoms include fever‚ hypotension
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