For the completion of this experiment the procedures were guided with the Rainbow Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells
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unknown was inoculated into a TSI slant by stabbing the specimen into the butt using a needle and then streaking on the surface of the agar. The sample was allowed to incubate for 24 hours and read for results. The next test was the citrate utilization slant which examines the ability for a bacteria to use citrate as the sole carbon and energy source. The citrate agar tube was inoculated with the bug by using a needle and then streaking over the surface of the slant. Upon an incubation period of 48
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Antibacterial activity of allopathic medicine versus phytomedicine against Enterococcus faecalis: An in-vitro study. Abstract Background: A successful endodontic treatment depends on appropriate instrumentation‚ irrigation‚ decontamination and obturation. Decontamination of root canal plays a crucial role in the success of endodontic treatment. Therefore‚ a suitable medicament is essential which can effectively eliminate bacterial contamination. Aim and objectives: To investigate and compare the
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sociological groups by employing interpretive and naturalistic approaches. It is both observational and narrative in nature and relies less on the experimental elements normally associated with scientific research (reliability‚ validity and generalizability). Agar (1980) suggests that qualitative inquiry relies more on appearance‚ verisimilitude and transferability. On the other hand‚ Bosk (2001) emphasizes the importance of credibility‚ transferability‚ dependability and conformability in qualitative studies
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Obtain agar plates from your instructor and label them as in the figure 1 provided below. 2. Using a sterile pipet‚ remove 130µL mixed bacterial suspension from the tube “C “‚ remove the lid from the control plate and dispense the bacteria unto the agar. Use a cell spreader to spread the bacteria evenly onto the agar surface. Use of the cell spreader; Dip the cell spreader into ethanol. Pass the spreader
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Floyd and Dr. Kennedy (1) unless otherwise noted. The first step that was used was a three streak method‚ described in Lab 4‚ on a Blood Heart Infused/Trypticase Soy Agar plate to isolate the two unknown bacteria. Once the two bacteria were incubated‚ grown‚ and isolated they were each individually streaked on a Trypticase Soy Agar plate to isolate individual colonies to be studied‚ tested and identified. After incubation of the individual TSA plates‚ the morphologies were viewed and noted and
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quantitative Ames test is similar to the spot-overlay Ames test‚ except that the mutant Salmonella tymphimuium (which cannot composite histidine) is treated with various concentrations of the mutagen Diet Coke to view which sections of the Davis Minimal Agar Plate form colonies (Bjeldanes‚ et al. 1982). Mutagenicity has been known to be carcinogenic and that can cause a toxicological effect that presents a potential health risk for humans ( Resende‚ et al. 2012). The purpose for doing this experiment
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the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size when determining the organism. Identify this type of agar. We use this agar to check for _______. Name This Technique. What is its purpose? What type of hemolysis is pictured below? Identify the Following I go straight from the 4x objective to the 100 x objective? Why? Identify the Following Identify the
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are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants also contains a pH indicator‚ phenol red‚ which can be used to test the presence of fermentation. If there is a
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Went’s experiment but the agar block was placed on one side of the plant and not in the middle. This caused the plant to grow curved. This is because the auxin went all to one side causing it to grow. One side growing faster then another causes the plant to bend. Gravity can cause an unequal amount of auxin. When a tip of a coleoptile is put on an agar block evenly both side of the block will get equal amounts of auxin but if you were to tip the 90 degrees one side of the agar block would receive more
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