The unusual members of NFGNB represent a challenge for the routine clinical microbiological laboratories‚ owing to their complex identification along with their unpredictable antimicrobial susceptibility patterns 14. The isolation rate of NFGNB (18.7%) recorded in our study comes in agreement with a previous Indian study carried out in a tertiary care hospital‚ where it was 16.18% 15. Whereas‚ it comes higher the result recorded in another Indian tertiary care hospital where it was 9.32% 16. The
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the streak‚ meaning that the organism could not use the starch and therefore no starch utilization. The Pseudomonas-F agar was viewed in the dark under UV light and fluoresced a yellow pigment‚ meaning production of pyoverdin and a positive on fluorescent pigmentation. The organism was capable of lipase production as the streak had a clear zone around it in the spirit blue agar‚ meaning the lipase reagent in the medium was degraded and utilized. The gelatinase test showed no clearing on the strip
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pUC18‚ respectively. Two LB/Amp agar plates were treated in the similar way. The GVM agar plates was inoculated with A.fischeri. Between each streak‚ the inoculating loop was sterilized by placing above the
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Microbial analysis of soil‚ of top layer from selected sites of Area near Dahisar River Saika N. Esani University of Mumbai (Email – saikae@ymail.com) Abstract: soil samples were collected fortnightly from area near Dahisar River‚ A river in suburb of Mumbai. laboratory analysis started from July 2010 to September 2010. Total bacterial and fungal count were estimated by standard spread plate isolation. Isolated bacteria were subject to colony characterization and were estimated by their
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Objectives To be able the students to differentiate and observe what the branded antiseptics and disinfectants can do. Methodology Materials and Procedures In order to start the activity‚ we cooked agar where we cultivated the bacteria (Staphylococcus epidermis). After plating and cooling the agar inside the petri dish‚ we foreplate the bacteria by using a cotton swab. We prepared two (2) petri dishes. One is for the bacteria and the other one is by swabbing the cotton swab to any surface and then
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biasanya dituangkan dalam suatu tindakan realistis yang digunakan untuk mencapai goal yang telah ditetapkan. Dalam dunia bisnis‚ jika suatu perusahaan ingin mencapai misi maupun visinya maka perusahaan tersebut harus memiliki daya saing strategis agar dapat bertahan di pasar yang kompetitif. Daya saing strategis (strategic competitiveness) dapat dicapai apabila sebuah perusahaan berhasil merumuskan serta menerapkan suatu strategi tersebut dan perusahaan pesaing tidak secara berkesinambungan menerapkannya
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CERTIFICATE ACKNOWLEDGEMENTS "There are times when silence speaks so much more loudly than words of praise to only as good as belittle a person‚ whose words do not express‚ but only put a veneer over true feelings‚ which are of gratitude at this point of time." I would like to express my sincere gratitude to my chemistry teachers Mrs. Meenakshi‚ Mrs. Sonali and Mrs. Shampa for their vital support‚ guidance and encouragement - without which this project would not have come forth. I would
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of incubation‚ results were collected as growth or no growth for each temperature. Methods and Materials: Figure 1a: agar plate Materials 6 nutrient agar plates media grown overnight in Tryptic Soy Broth E. coli P. fluorescens B. stearothermophi lis Inoculum loop
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ii) Tube of sterile distilled water. iii) Petri dishes with nutrient agar iv) China Marker v) Object to be tested (all are from the front of the class room): (1) Teachers Chair (2) Computer Keyboard (3) Computer Mouse (4) Teachers Desktop b) Method: vi) draw‚ with a china marker‚ on the outside of the agar portion‚ not the lid‚ of a Petri
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with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin). The extracted plasmid DNA is important as it contains ampicillin-resistant gene. As such‚ E. coli cells that have taken up this plasmid DNA will be resistant to ampicillin and survive‚ hence growth of colonies will be observed on the agar plates. One of the rationales behind heat shock method is to create pores‚ allowing uptake of plasmid DNA (Panja et
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