count) of the total viable bacteria in the rice salad on a general non-selective agar using either the pour or the spread plate method. To confirm that the outbreak had been caused by any B. cereus present in the rice salad a selective media agar‚ such as mannitol egg yolk polymixin agar (MEYP/MYP)‚ should be used. Once B. cereus has been confirmed a further enumeration of the B. cereus should be performed on the MEYP/MYP agar selective media plate to show whether the amount of B. cereus present is within
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since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide. Fixing = passing the smear through the flame of the laboratory burner three times in rapid succession
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used to test antimicrobial activity against disease causing bacteria Staphylococcuaureus. Extracts of varying concentrations of Azadirachtaindica fruit pulp and leaf extract were prepared using Phosphate Buffer and tested against test organisms using agar diffusion method. Oxfloxacinof same varying concentrations were used to compare the effect of antimicrobial activity of fruit pulp and leaf extract. Keywords: Azadirachtaindica‚ Antimicrobial activity‚ Staphylococcus aureus. I. INTRODUCTION Azadirachtaindica
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CHAPTER ONE 1.0 INTRODUCTION Natural rubber is produced by over 2000 plants species and its main constituent is poly (cis-I‚4-isoprene).a highly unsaturated hydrocarbon. Since 1914 there have been efforts to investigate microbial rubber degradation: However‚ only recently have the first proteins involved in this process have been identified and characterized and have the corresponding genes cloned. Analysis of the degradation product of natural rubber and synthetic rubbers isolated from various
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plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate and isolate this microorganism from the many other microorganisms with which
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_____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform pore size than that of agar. It is also easily poured and does not denature the samples. In agarose gel electrophoresis‚ DNA or RNA fragments are separated or isolated according
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A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot
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ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and
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(positive). The catalase test result indicated that my unknown was negative because no bubbles formed when I placed a loop of the organism into hydrogen peroxide. The next step was to examine a blood agar plate to examine the colonial morphology of hemolysis ‚ my organism produced gamma hemolysis on the blood agar because there was no hemolysis on the plate after 48 hours incubation period. After examining my unknown for hemolysis I set a series of five experiments: Bacitracin SX CAMP Enterococcosel
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for antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚ was spread on the same plate and reincubated
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