INTRODUCTION Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria‚ yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method
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TSI test because there is no pink color for Urea test and no black color on the bottom of the tube in TSI test. Then‚ follow three tests results are all positive. That is deep blue color for citrate test‚ clear zone surrounding growth for skim milk agar
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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ATCC 8007. The bacterium was activated in Man-Rogosa-Sharpe (MRS) agar medium (Difco‚ BD Diagnostic Systems‚ Maryland‚ USA) consisting of (g L1): peptone casein‚ 30; meat extract‚ 10; yeast extract‚ 6.0; sodium acetate‚ 5.0; ammonium citrate‚ 2.0; glucose‚ 0.2; magnesium sulfate‚ 0.2; manganese sulfate‚ 0.05; dipotassium phosphate‚ 2.0. The pH of the medium was adjusted to 6.5. The master cell bank culture was inoculated into agar plates and incubated for 48 h to produce the working cell culture
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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the height. * Diffusion in solids -4 agar plates -KMnO4 crystals -methyl orange -refrigeration -ruler The experiment requires 4 plates of agar due to his transparency and the colloid that forms when mixed with water. One pair of plates is labeled 4^0 C and the second pair RT (room temperature). After taking the lids of the first pair ‚using a tooth pick‚ a small crystal of potassium permanganate is placed in the center of the of the agar and the same amount of methyl orange. The
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SEROLOGICAL‚ CULTURAL AND MOLECULAR DETECTION OF BRUCELLA INFECTION IN BREEDING BULLS A THESIS SUBMITTED TO THE ANAND AGRICULTURAL UNIVERSITY IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF Doctor of Philosophy IN VETERINARY MICROBIOLOGY BY AMIT N. KANANI M. V. Sc. DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND-388001 (GUJARAT) 2007 Reg. No. 04-05194-2001 Dr. J. H. Purohit
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CHAPTER I INTRODUCTION This chapter presents the background of the problem‚ main problem‚ the sub-problems‚ and hypothesis‚ significance of the study and the scope and limitations of the study. Background of the Problem Nile Tilapia (Oreochromis niloticus) is a very popular aquaculture species in the Philippines at present and considered as an “aquatic chicken” offering economical and social benefits mainly for rural communities. It also play vital role in terms of worldwide employment‚ however
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Testing on the effectiveness of anti acne gel (Acne vulgaris) mild and moderate degree acne from ethanol extract of galanga (Kaemferia galanga L.) in students at Miftahus Salam Boarding School in Banyumas Yenni Bahar*‚ Berna Elya*‚ Mahdi Jufri* Faculty of Pharmacy‚ University of Indonesia‚ Depok 16424‚ Indonesia Coresponding author : yennibahar@gmail.com ABSTRACT Acne is an aesthetic skin disorder‚ usually in adolescents‚ with a clinical manifestation of comedones‚ papules‚ pustules‚ and nodules
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Walter A. Shewhart March 18‚ 1981- March 11‚ 1967 Walter Andrew Shewhart is regarded by many as the “father of statistical quality control.” He was born in New Canton‚ Illinois on March 18‚ 1891 to Anton and Esta Barney Shewhart. Walter attained his bachelor’s and master’s from the University of Illinois in physics and graduated with is PhD from UC Berkely in 1917. Throughout his adulthood‚ he was a professor at both universities and ran the physics department at the Wisconsin Normal School
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