LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
Premium Plasmid Transformation Escherichia coli
Lab 5 The Diffraction Grating Chinua McDonald Objective: To measure the wavelength of light with a diffraction grating. Theory: The two types of diffraction gratings are the transmission and reflection gratings. They are made by ruling on a piece of glass or metal a number of evenly spaced lines with a fine diamond point. Diffraction phenomena can be analyzed in terms of Huygens’ principle‚ according to which every point on the wave front of a wave should be considered as a source
Premium Light Diffraction Wavelength
indicated by a very pale pink color. To calculate the molarity of NaOH‚ the following equation was used MNaOH x VNaOH = MKHP x VKHP therefore the molarity was .125 M. INTRODUCTION This lab experiment covers the preparation of standard solution and the acid/base titration. The first part of the lab is to prepare a standard solution of Potassium hydrogen per. A standard solution is a solution of known concentration‚ in which it is prepared using exacting techniques to make sure that the molarity
Premium Sodium hydroxide Titration
February 20th‚ 2014 Lab report 4 Abstract This pBlu lab had for purpose to present the changes of the strain of E. coli bacteria due to new genetic information being introduced into the cell. In this experiment we are freezing and heat shocking the E. Coli bacteria that is then forced to take the plasmid DNA. The E. coli then transforms the pBLu plasmid‚ which carries the genes coding for two identifiable phenotypes. After following the Carolina Biological steps our lab worked well and we able
Premium Sociology United States Family
Moment of Inertia and Rotational Motion Garret Hebert PHY 2311 Tues 1:00 garret.hebert@hindscc.edu Abstract: During this lab we will study what rotational Inertia is and how different shapes of masses and different masses behave inertially when compared to each other. We will specifically study the differences of inertia between a disk and a ring. We will use increasing forces to induce angular acceleration of both a disk and a ring of a certain mass. We will then then measure the differences
Premium Classical mechanics Inertia Torque
cell fragility - Osmotic Hemolysis. Retrieved on July 4 2013 from‚ http://www.medicine.mcgill.ca/physio/vlab/bloodlab/eryfrag1_n.htm Reece‚ J.B. et al. (2011). Campbell biology (9th edition). Harlow: Pearson Education‚ Inc. Transport across- Membrane Lab‚ Retrieved on July 4 2013 from‚ http://course1.winona.edu/mgarbrecht/Membrane%20Lab.pdf Why Does Milk Form a Skin When it is Heated?‚ Retrieved on July 4 2013 from‚ http://www.wisegeek.org/why-does-milk-form-a-skin-when-it-is-heated.htm
Premium Osmosis Cell membrane Red blood cell
Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential
Premium Gram staining Staining Microbiology
or magnesium environment will be significantly different than the rate of streaming in plain 2% agar and that calcium and magnesium will have an equal effect on cytoplasmic streaming in Physarum polycephalum because of their similar chemical properties. Methods: To set up P. polycephalum samples‚ 15 plates of agar were set up to culture the mold. After mixing 2M solutions of calcium and magnesium‚ agar was measured and mixed into the solutions and 5 plates of calcium solution‚ 5 plates of magnesium
Premium Magnesium Standard deviation Cell
cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
Premium Bacteria
microbial world so that the benefits to the Earth and humans are known(6). This lab specifically does not require the knowledge of what each organism does when introduced to another living organism‚ like a human. Figuring out the type of bacteria could help with further experiments. The idea of the unknown bacteria lab is to show
Premium Bacteria Microorganism Organism