PSBPP103 Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes
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Lab 5: Bacteriophage 5 February 2013 Introduction: A bacteriophage is essentially a virus that specifically infects bacteria. A bacterial cell infection progresses in much the same way as a eukaryotic cell. A plaque forms when a bacterial cells growing on soft agar burst from the viral infection and appears like a hole in the agar. Each plaque is created by the progeny of an individual phage and can thus be counted to determine the number of phage particles in a sample. The purpose of this
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group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria on it to see if the bacteria obtained is gram positive or gram negative. My hypothesis is there will be a lot of bacterial growth on all of the plate. Materials and Methods -Petri dish containing nutrient agar -Cotton swabs -Sharpie A Petri plate containing nutrient agar was used in the
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that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed by a bacteria to improve the lives of people. Transformation happened when a gene is transferred from one bacterium to another one on a plasmid. E. coli is the organism that genetics prefer to
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Kristen Magliola 9/9/13 BIO: 414L Lab Report: SOC Medium I. Purpose: To make liquid and solid media for microbial growth II. Background: There are four different types of media used to grow microbes. They consist of Enriched media‚ Nutrient media‚ Selective media‚ and differential media. Enriched media is a growth media supplemented with complex biological molecules. This type of media is used for blood‚ coagulated blood‚ amino acids‚ etc. Nutrient media provides nutrients necessary
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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collected in lab #1 shows the relationship of surface areand volume in the artificial cells to the diffusion rate using the phenolphthalein-NaOH agar and the HCl solution. Lab #2 was a model of diffusion and osmosis‚ in which we filled the model cells with different solutions and determined the rate of diffusion. In lab #3‚ the results demonstrated the interactions between selectively permeable membranes‚ water‚ and solutes and how they are important in cellular and organismal functions. In lab #1‚ the
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Since the Grignard reagent can easily react with water‚ all glassware including the 25 ml round bottom flask‚ magnetic stir bar‚ 3 and 5 ml conical vial‚ 50 mL Erlenmeyer flask‚ claisen adapter‚ drying tube and 5 glass pasteur pipets were first added to a 250mL beaker and placed in the oven for 30 minutes. After the completion of the thirty minutes‚ 0.150 g of shiny magnesium turnings and a stir bar was first added to the round bottom flask and the claisen adapter along with the drying tube packed
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Diffusion & Cell Size Lab Background The absorption of nutrients‚ excretion of cellular wastes‚ and the exchange of respiratory gasses are life processes which depend upon the efficient transport of substances into‚ out of‚ and throughout living cells. The process of diffusion can be easily visualized by adding a drop of blue food coloring to a glass of water. Initially‚ the food coloring remains in a small area in the water‚ dying it a dark blue. Over time‚ the molecules of food coloring
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Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
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