unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours. Upon observing the growth on this plate‚ it is fairly obvious that one of the organisms is Serratia marcescens
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Quantitative & Semi-quantitative Viable Plate Counts Molly Wright‚ Jenny Cano‚ Rosa Ramirez BIOL 2420 Lab M/W 4/20/15 Professor Rotibi ABSTRACT: Accurate evaluation of bacterial colonization as a predictive index for alfalfa sprouts has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of the alfalfa water. In this study 1 package of alfalfa sprouts were cultured by a semi-quantitative technique that enumerated
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Bacteria Lab Write-Up Jianna Liu P8 Purpose: The purpose of this lab is to find out which substance‚ alcohol‚ antibacterial soap‚ water‚ or hydrogen peroxide‚ is the best at preventing the growth of bacteria. Hypothesis: Antibacterial soap will be the best because soap is a substance that people use to wash their hands because it takes away the germs. Even the name‚ antibacterial soap‚ suggests that it fights off bacteria. Hydrogen peroxide is second because it is used
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The Effect of Disinfectants and Antiseptics on Bacteria Growth Abstract The purpose of this lab was to determine the effect of disinfectants and antiseptics on bacterial growth. By observing the effects of 5 different inhibitors including alcohol‚ bleach‚ soap solution and distilled water‚ it was determined what antiseptic or disinfectant was able to best inhibit this kind of bacterial growth. Nutrient Agar was poured into a Petri dish with four quadrants and then a pipette was used to place bacterial
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Escherichia coli Microbiology Lab NAS 185-14L Introduction In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is
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bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface of Simmon’s citrate agar. Followed by overnight incubation at 37°C. Day after the slant
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Genetic Variation in Sordaria Finicola Introduction: The purpose of the Sordaria Lab was to explore the affects of genetic variation caused by meiosis and to record how sexual reproduction] affects the amount of crossing over in certain strains of Sordaria Fimicola. These organisms are ascomycetes and are also known as sac fungi. This is because the shape of their asci is in the form of a sac; inside each sac there are structures called ascospores. It is these structures‚ ascospores‚ where genetic
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Computer Lab Rules & Regulations Electronic workstations may only be used by current Swinburne University students and staffs. Swinburne identity card must be presented on request. Swinburne computing facilities should only be used for educational‚ research and administrative purposes of Swinburne. All other uses are strictly prohibited. The following rules and terms apply to all computers on campus. Terms and Conditions 1. All users must abide by the license requirements of any software
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unknown mixture‚ using a number of laboratory tests. Over the semester‚ multiple tests were carried out to identify between gram-positive and gram-negative bacteria including the Mannitol Salt Agar (MSA) media test‚ which selects only for gram positive bacteria‚ and the use of Eosin Methylene Blue Levine (EMB) Agar media‚ which selects for gram negative bacteria and differentiates between lactose fermenters (paracolons) and non-lactose fermenters (coliforms).These tests along with other selective and/or
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nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of each petri dish and evenly spread bacterial culture around the agar plate. Cover and allow the culture to soak into agar for at east 15 minutes. Using sterile forceps‚ carefully place one filter disk from designated sample into the middle of each
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