Research Plan Title: In vitro Antibacterial Activity of Santol (Sandoricum koet jape) at Different pH of Agar School : Ramon Magsaysay (Cubao) High School School Address: Ermin Garcia St.‚ Cubao Quezon City Research Adviser: Mr. Ryan D. Balandra Statement of the Problem The aim of the study is to identify the effect of different pH level of the Agar plate to the antibacterial activity of Santol (Sandoricum koet jape). Specifically‚ the study will seek for the answer of the question:
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diffusion rate in agar cubes? Hypothesis: The rate of diffusion in directly related to the surface area to volume ratio of cells and is responsible for the efficiency of absorbing nutrients‚ oxygen‚ minerals etc. in the cell. This ratio is specific to cells as they require a ratio that isn’t big enough to take too long to receive the nutrients and oxygen or too small to impair diffusion‚ known as the maximum sustainable cell size. Due to this it would be expected that the smaller the agar cube the larger
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when the agar blocks are placed in solutions of different concentrations of HCl. As the concentration of HCl increases‚ the rate of diffusion will also increase due to the steeper concentration gradient created. To investigate the change in rate of diffusion when different concentrations of HCl are used‚ I used agar blocks that are stained with universal indicator that would change from green to pink when exposed to HCl. To prepare the agar blocks‚ I used knife and ruler to acquire 5 agar blocks with
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plates. This indicates that the Tn10 exposure was the cause of mutant variation. There were ten hypothesized auxotrophic colonies based on the comparison of mutagenized colonies in minimal and nutrient agar. These ten colonies were confirmed to be auxotrophic by their lack of growth in the minimal agar replica plate. The required growth factors for each auxotroph were determined based on which pool plates fostered growth for each streak. Auxotrophs 1‚ 2‚ 3‚ and 4 require valine as a growth factor
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The product was transformed from the traditional masculine bar and given a more curvy look. The carbonic smell was substituted by perfume fragrance. The focus now was on a complete bathing experience for the family and the new tagline “kya aap lifebuoy se nahaye kya” did complete justice to the cause. After this re-launch‚ Lifebuoy’s market share in the bath soap sector rose from 12% to 18%. 2002 also saw the launch of “LIFEBUOY SWASTHYA CHETNA” program aimed to educate people in rural India about
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nutrients” with illustrations (within 150 words). Bengali (3rd Language) 1. Write an essay on Summer season with illustrations. Hindi (2nd Language) 1. Har Saal Mausam ka badalta mijaj varnan karo. Hindi (3rd Language) 1. Prakriti mein apne kya kya dekha. (Panch chitra banakar unkey nam likho. Mathematics Worksheet 3‚ 5‚ 6 and 7 From Me and Mine. Geography 1. Collect pictures according to classification of natural resources as shown on Pg. 2 of your text. Make a collage with the pictures
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Equipment: * 3 agar plates * Blank discs * Antibiotic assay disc * Culture of Bacillus subtilis * Forceps * Bunsen Burner * Inoculating loop * Tea Tree oil * Heat Mat Method: First Agar Plate * Sterilise inoculating loop using Bunsen burner‚ allow inoculating loop to cool before using inoculating loop to pick up‚ the liquid of the culture. * Agar plate to be open as little as possible‚ minimising
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of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth. (1) 2(A). Experiment 1: Choose 2 words that describe the appearance of the pre –incubation broth: (i) Straw yellow
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Hypothesis: The hypothesis for this particular practical experiment is that‚ as the pH decreases or increases from neutral pH of approximately 7‚ the amount of microbial growth on the agar solution will decrease Materials: Agar Solution Preparation: *hand sanitizer/ hand wash soap *small container *1g of agar powder *0.25g of beef extract stock *60mL of heated water *electronic balance *small spoons (2x) *teaspoons (2x) *0.2M Acetic Acid Solution (pH of 3) *0.2M Sodium Hydroxide Solution
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organisms can easily be seen using differing types of agar‚ which creates an ideal environment for the organisms to form colonies‚ which are groups of hundreds of organisms that can be seen with the naked eye. In order to see individual microorganisms‚ it is necessary to use the magnification of a high-powered microscope. These techniques of microbiology are used in the following five experiments. The first experiment used Trypticase Soy Nutrient Agar (TSA)‚ which can grow a wide variety of organisms
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