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    O157 Case Study

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    designed Rainbow Agar O157 for the isolation and identification of EHEC. In this medium E. coli O157 was characterised by black colonies whereas O113 and some other EHEC strains were mauve‚ red or pink and indistinguishable from other strains of E. coli. Manafi and Kremsmaier (2001) evaluated the efficiency of different media for detecting Escherichia coli O157:H7. They found that SMAC agar had a sensitivity and specificity of 94.1 per cent and 91.6 per cent‚ Fluorocult HC agar

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    correct‚ I carried out an experiment where I grew bacteria on 14 different agar plates and placed garlic on seven of the agar plates and then had no garlic present in the other seven agar plates. All 14 agar plates were kept under the same conditions while the bacteria colony was growing in order to ensure that all factors were kept constant. From the results‚ it was evidently clear that the size of the bacteria colony of the agar plates without garlic with an average of 115.29 mm² was much greater than

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    causative bacteria or fungus. Potato dextrose agar is a good nutrient agar for mycelia to thrive on which is present in most fungal moulds.1 Standard nutrient agar is a general utility used for non-fastidious microorganisms.2 Aim The aim is to isolate fungi and bacteria colonies from diseased and healthy leaves. Materials and Methods Materials used for the experiment was two of each: standard nutrient agar plate and potato dextrose agar plate. To remove any epiphytic or saprophytic

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    turn the agar plate over and divide the plate into four quadrants and label the agar plate whether you used the E. coli or B. megaterium and number the quadrants 1 through 4. Please keep in mind that one pair will test the E. coli and Environment 1 or 2‚ and one pair will test B. megaterium and Environment 1 or 2. After‚ you will need to swab the E. coli and B. megaterium on two different nutrient agar plates using a sterile disposable inoculating loop. Remember not to dig in into the agar or the

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    Place Domino’s entered India in 1996 through a franchise agreement with Vam Bhartia Corp. The first outlet was opened in Delhi. With the overwhelming success of the first outlet‚ the company opened another outlet in Delhi. By 2000‚ Domino’s had a presence in all the major cities and towns in India. By March 2000‚ Domino’s opened 37 outlets all over India. Between April 2000 and February 2001‚ Domino’s set up 64 more outlets in India. Delhi had the maximum number of outlets – 17‚ followed by Mumbai

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    Totipotency of Plant Cell

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    INTRODUCTION Background Totipotency is the ability of a single cell to divide and produce all the differentiated cells in an organism‚ including extraembryonic tissues. Totipotent cells formed during sexual and asexual reproduction include spores and zygotes. Zygotes are the products of the fusion of two gametes. In some organisms‚ cells can dedifferentiate and regain totipotency. For example‚ a plant cutting or callus can be used to grow an entire plant. Human development begins when a sperm fertilizes

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    Title: What is the effect of concentration of acid on the rate of diffusion in agar blocks? Aim: To investigate how the concentration affects the rate of diffusion of hydrochloric acid through agar blocks Research Question: To determine how will different concentrations (0.1M‚ 0.2M‚ 0.3M‚ 0.4M‚ 0.5M) of hydrochloric acid affect the rate of diffusion of sodium chloride through agar blocks? Introduction-include prediction; information you have researched before Diffusion refers to the passive movement

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    UNIVERSITY OF DAR –ES-SALAAM COLLEGE OF NATURAL AND APPLIED SCIENCES DEPARTMENT OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY MC206: FOOD MICROBIOLOGY PRACTICALS PRACTICAL 1 MICROORGANISMS IN THE ENVIRONMENT GROUP #:1 NAME: DUSENGEMUNGU Léonce REG #: 2011-04-07086 COURSE INSTRUCTOR: Dr Mugassa S.T Rubindamayugi

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    source. Simmon’s citrate agar will be prepared by using Oxoid well-prepared Simmon’s citrate agar powder. The Simmon’s citrate agar was prepared by using Oxoid well-prepared Simmon’s citrate agar powder. The preparation was began by suspending 5.75g of the powder in 250 ml of distilled water and mixed thoroughly. The following shows the amount of each composition in 1 litre of Simmon’s citrate agar solution prepared using Simmon’s citrate agar powder. This Simmon’s citrate agar solution was then heated

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    patients such as those suffering from cystic fibrosis or immunocompromised individuals. MIC methods are varies such as disc tests that give qualitative result‚ dilution tests which testing the ability of microorganisms to produce visible growth on several agar plates. It containing dilutions of the culture and antimicrobial agent.

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