was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were identified as Staphylococcus aureus with a positive confirmatory test‚ mannitol salt agar‚ showing consistent results as well for S. aureus. The Gram-negative bacteria were Pseudomonas aeruginosa with a positive confirmatory
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distending the cell Bacillus cereus Table 2. The observation of the colonies on the nutrient agar plates after incubated Three isolation techniques were used; streak plate‚ spread plate and pour plate and the agar plates were then inverted and incubated at 370C for one day. The distributions of colonies were then observed. Observations were recorded. Isolation Techniques Observation on the nutrient agar plates Streak plate At the first inoculum‚ all the bacterial colonies were overlapping with each
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of a wide variety of microorganisms whilst the agar function is the solid media onto which the bacteria can be isolated as independent colonies which are representatives of different bacterial species. Controlling microbial growth is necessary in numerous situations and is greatly significant in areas such as medicine. Growth of microorganisms is
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Abstract Microorganisms are plentiful and widespread in the environment. In this lab‚ we undertook to determine the differences in the agars being used and the different colony count observed. After taking four different samples of microbes from the environment and swabbed them in two different plates one with nutrient agar and the other with sabouaud dextrose agar. After the microbes had incubated for 48 hours no results were discovered from the swabs we had taken from the environment. This lab
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An agar plate is a Petri dish that contains a combination of agar and nutrients that help microorganisms grow. The proper method of setting microorganisms on an agar plate is know as “streaking”. In order to streak‚ the microorganisms are placed on a sterile swab or metal wire‚ which is then dragged lightly against the agar solution‚ leaving behind the microorganisms. The amount of organisms is greatest at the beginning
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dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod‚ culture was taken from the tube and dragged across area 1 several time‚of the agar. The agar was then turned 90º‚ and the loop was flamed and cooled. Taking some culture from area 1‚ it was dragged over area two‚and the same steps were done for areas 3 and 4.Another technique used was spread-plate‚ where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod. The rod
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or magnesium environment will be significantly different than the rate of streaming in plain 2% agar and that calcium and magnesium will have an equal effect on cytoplasmic streaming in Physarum polycephalum because of their similar chemical properties. Methods: To set up P. polycephalum samples‚ 15 plates of agar were set up to culture the mold. After mixing 2M solutions of calcium and magnesium‚ agar was measured and mixed into the solutions and 5 plates of calcium solution‚ 5 plates of magnesium
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Introduction In my report I will discuss what I did as an experiment and what I hope for it to achieve. Firstly I carried out an experiment to assay the effectiveness that a range of disinfectants have on the growth of ecoli and whether or not it can prevent it from growing. From the experiment i should be able to see that some disinfectants have a greater effect than others do. From this I shall then draw a conclusion and evaluation on what was the most effective‚ and could there have been any
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nitrate ions (NO3-) with metal ions. The most common oxidizer is potassium nitrate‚ which decomposes to potassium oxide‚ nitrogen gas‚ and oxygen gas. Oxidantii utilizati cel mai frecvent sunt nitratii‚ componenta majora a pulberii negre. Nitratii sunt compusi din ioni nitrati (NO3-) cu ioni metalici. Oxidantul cel mai comun este azotatul de potasiu care se ddescompune in oxid de potasiu‚ azot‚ oxigen/ When reacting‚ nitrates release two of their three oxygen atoms. Because
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count) of the total viable bacteria in the rice salad on a general non-selective agar using either the pour or the spread plate method. To confirm that the outbreak had been caused by any B. cereus present in the rice salad a selective media agar‚ such as mannitol egg yolk polymixin agar (MEYP/MYP)‚ should be used. Once B. cereus has been confirmed a further enumeration of the B. cereus should be performed on the MEYP/MYP agar selective media plate to show whether the amount of B. cereus present is within
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