be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen. Hypothesis: For this experiment‚ antibodies will be placed in wells on an agar plate‚ surrounded by wells containing serum (proteins‚ antigens etc.). If the antibody interacts with the antigen of surrounding wells‚ then the presence
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IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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diffusion Materials: Agar‚ Ruler Chemicals: Potassium Permanganate‚ Methylene Blue and Potassium Dichromate. Method: Obtain a pinch of potassium permanganate‚ methylene blue and potassium dichromate. Place each substance carefully on the surface of agar‚ carefully noting which is which so that the crystals are spread equally over the agar and not too close to the edge of the dish. Measure the diameter of the colored area immediately after adding the substance to the agar with regular 10-minute
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for their industrial production are Bacillus subtilis‚ Bacillus licheniformis‚ Bacillus amyloliquifaciens and Aspergillus niger General Lab Requirements: • Autoclave or pressure cooker • Hot Plate or Microwave oven • Nutrient Agar powder • Potato Dextrose Agar powder • Soluble starch • Weighing scales • Shaker • Spectrophotometer or colorimeter • Water bath (Temperature controlled) Materials per group of 4 students • Hand trowel or disposable spoons • Sterile pipettes (One each of
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of LB agar. We then put 250 µn of CaCl2 transformation solution into two micro test tubes for the purpose of changing the bacterium’s cell wall to allow the pGLO plasmid to enter more easily. Second‚ we placed one colony of E. Coli from our original plate into each of the micro test tubes. Subsequently‚ we extracted a loopful of pGLO plasmid with a sterile loop and then placed it in one of the micro test tubes. We incubated the tubes on the ice for ten minutes while we prepared four new agar plates
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(positive). The catalase test result indicated that my unknown was negative because no bubbles formed when I placed a loop of the organism into hydrogen peroxide. The next step was to examine a blood agar plate to examine the colonial morphology of hemolysis ‚ my organism produced gamma hemolysis on the blood agar because there was no hemolysis on the plate after 48 hours incubation period. After examining my unknown for hemolysis I set a series of five experiments: Bacitracin SX CAMP Enterococcosel
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that will be of use to you throughout your biological work. Procedure 1. Heat the test tubes of sterile agar medium in the water bath until the agar melts. 2. Remove the test tubes from the water bath. Let them cool enough to hold in your hand‚ but not so much that the agar becomes solid again. Perform the following transfer as quickly as possible. You must work rapidly so that the liquid agar will not cool and solidify before the transfer is completed. 3. Hold both a test tube
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microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol salt agar‚ blood agar and MacConkey agar plates which must be used based on the components of the bacteria. The catalase test will be used to understand the difference in facultative anaerobic and groam positive from aero tolerant anaerobes. The coagulase test converts fibrogen
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the growth of oral bacteria. Each procedure consisted of three trials for each test subject. For procedure #1‚ saliva was collected from each test subject and then mixed in tubes containing melted nutrient agar. Each tube was labeled with the test subject#s initials and trial number. The agar
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microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized‚ a flame sterilized inoculating loop will be used to gather
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