semi-permeable outer membrane of E. coli would protect the bacteria from any antimicrobial properties of cilantro. A drop of cilantro juice and water in varying concentrations (1:10‚ 1:20‚ 1:40‚ 1:80) was added to a nutrient agar plate inoculated with S. epidermis and a nutrient agar plate inoculated with E. coli. The plates were incubated for 48 hours and then observed for a zone of clearing where the cilantro juice drop was placed. Cilantro was found to not display antimicrobial activity against either
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sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate. 5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate‚ making sure the lid of the culture container does not touch the surface‚ and that the lid of the agar plate is quickly replaced after this step has been carried out. 6. Sterilise the grabbing part and up the handle
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Driss Chraïbi‚ explore un seul personnage‚ la mère. Mais le roman‚ n’est pas une étude de caractère ; c’est plutôt une étude d’un pays. Le style et ton de Chraïbi varient partout dans le roman‚ mais la texte reste toujours plein des métaphores et sarcasme. Il y a des temps quand l’intrigue vire complètement hors de réalité‚ mais d’autres fois ou les circonstances sont vraiment plausibles. Chraïbi approfondie a la fois l’évolution de la mère‚ mais aussi l’évolution de sa famille‚ et le monde entier
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There are two stone sculpture from the New Kingdom. One is in raised relief and of a man named Mai and his wife Urel. These two are not royalty but are relatives of a man named Ramose and this work is from his tomb. They are part of an image of guests seated at a banquet. The second is a sunk relief sculpture of a king from this same time period named Akhenaten. We see him here with his wife Nefertiti and his three daughters‚ his son was not in this sculpture. This essay focuses on why the stone
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with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli EMB- pink mucoid colonies -------- K. pneumoniae e.coli (left) k.pneumoniae (right) *TSI ALK/Ag-----Serratia marcecens‚ Salmonella paratyphi A red slant‚ yellow butt with cracks or agar displacement or bubbles if with:
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Day 1 Throughout the semester‚ I have learned multiple techniques for identifying bacteria. Learning how to gram stain‚ use specific media such as MacConkey agar‚ and test antibiotics to see which antibiotic would react best against a specific organism. All these techniques helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive
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cells growing on soft agar burst from the viral infection and appears like a hole in the agar. Each plaque is created by the progeny of an individual phage and can thus be counted to determine the number of phage particles in a sample. The purpose of this lab is to employ a plaque assay method to determine the number of infected phage particles in the given sample. Method & Materials: * (6) BHI plates at 37˚C * (5) tubes of 9.0mL TSB broth * (6) tubes of soft agar in a 50˚C water bath
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cold CaCl2 solution. Label one tube with your initials and a (+) and the other tube with your initials and a (-). 2. Transfer 2-4 large colonies using a sterile plastic loop to each microcentrifuge tube and completely resuspend. Do not transfer any agar. Put the tip of the loop into the CaCl2 solution and spin until there is not any cells on the loop. 3. Close each of the tubes and put them in ice. 4. Ask your teacher to
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Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
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Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA were used to inoculate Gram-positive tests‚ where as MacConkey Agar colonies were used to inoculate Gram-negative tests. Once the colonies were isolated and the appropriate Gram-negative and Gram-positive tests were conducted‚ the identification of both unknown organism were fairly easy. The results from the
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