pyogenes. The sputum sample was first inoculated in the agar plates MAC‚ SBAP‚ CHOC and also a direct gram stain of the sample was performed. After a day of incubation‚ the MAC agar gave a negative result‚ therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth of colonies. The CHOC agar had a mixed formation of colonies (gray colonies and 2+ tan colonies)‚ so it was isolated in another CHOC agar and it was mixed again. A gram stain procedure was done
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dish of nutrient agar‚kept led on ‚and used the marker to divided the petri dish ‚into teo sector. -opened lte lid slightly‚inoculate sector number 1 with thumbprint. -Taked sterial wire loop to transferred colony from a broth to an agar plate. -The loop should be cooled in the air for 10 to 20 sec and placed on the line drawed. -Remove a sample from a broth culture by using a sterile wire loop. -Transferred the sample from borth to agar plat. - When inoculated an agar plate‚ drawed the
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I removed he swabs from their packets and swabbed the inside of my cheek and put them back in their packets. 3. I then lifted the lid of the TSA plate slightly at an angle to prevent airborne microorganisms from contaminating the agar and smeared the surface of the agar with the swab from the floor and labelled it ‘TSA floor’. I did the same for the swab from my cheek and labelled it ‘TSA cheek’. I repeated this process for the SDA
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plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate and isolate this microorganism from the many other microorganisms with which
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shigellosis include Btilliant Green Agar‚ and Triple Sugar-Iron Agar. Expected results in a confirmed case of shigellosis are as follows: Brilliant Green Agar – Isolated Shigella colonies which do not ferment lactose or sucrose and appear red or white in color with no growth to trace growth on the Agar plate will be present. Triple Sugar-Iron Agar – Presence of Shigella will manifest as a red slant with a yellow butt with no H2S present. In Brilliant Green Agar‚ E. coli O157 would present as isolated
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temperature as temperature is lower their growth is also slower and temperature above 30ºC may cause mutation in petite and sporulation is highly inhibited. 6. Isolating Single Colonies: Done by streaking cultures out on an agar plate to dilute the cells on the surface of the agar‚ overlapping streaks‚ separation or distribution of cells to form isolated single colonies. 7. Estimating the Number of Yeast Cells in a Culture: How to differentiate between molds and yeast culture: Traditional methods:
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with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism. A gene is a piece of DNA that instruct for making
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the way physicians’ control/treat infectious disease within our bodies. In this lab‚ chemotherapeutic agents were evaluated by the disk-diffusion method. Chemotherapeutic agents are placed on the surface of a Petri plate containing Mueller-Hinton agar‚ which allows the agents to diffuse freely‚ and a growth medium is incubated over its’ surface. During incubation‚ the chemotherapeutic agents move from levels of high concentration‚ to low levels of concentration. To be effective‚ the agent must inhibit
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Experimental Methods In this lab‚ we use the procedure from Chemically Defined‚ Complex‚ Selective‚ and Differential Media. We divide the agar plates that have the unknown in third‚ write our initials‚ and date on the bottom of the agar plate. We also divide the agar plates into quadrants‚ label each organism in their quadrant‚ write our initials‚ and date on the bottom of the agar plates. We flame the loop and let it cool for a minute‚ we remove the cap of each culture tube of organisms with our pinky while
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ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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