investigated compounds were dissolved in DMSO at 10 and 20 mg ml-1‚separately [19‚21‚25‚27‚34]. Nutrient agar was prepared‚ then sterilized in an autoclave and poured in sterile Petri plates. After cooling of nutrient ager in Petri plates‚ the studied organisms were grown on agar. After that‚ the sterili Paper discs of Whatman saturated with solution of investigated compounds were placed in the agar by working holes using a sterile crook borer. These Petri dishes were incubated for 24 h at 37oC [25]
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Business Communication 2011 Why Indian Movies Are Being More Popular Among The Young Generation Than Bangladeshi Movies? BANGLADESI & INDIAN MOVIES RESEARCH Lightening Stars AMERICAN INTERNATIONAL UNIVERSITY-BANGLADESH Title Page Why Indian Movies Are Being More Popular Among The Young Generation Than Bangladeshi Movies: A Survey Among Young Generation
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dye‚ all of which inhibit the growth of gram-positive bacteria. As the name suggests‚ SS agar should theoretically only allow the growth of Salmonella and Shigella. In fact‚ many gram-negative bacteria cannot grow on SS agar. However‚ some enteric bacteria are able to grow on this medium‚ due to shared characteristics with Salmonella and Shigella‚ though their proliferation is limited. Both EMB and SS agars are also differential as they contain lactose‚ which causes indicator dyes in the media to
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Autumn White Biology 258-05 November 26‚ 2012 Unknown #19 Enterbacteriaceae Enterobacter aerogenes ABSTRACT The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks‚ ten tests were performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the
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Lab Practicum‚ Micro 200‚ study guide When you did your hand wash experiment‚ you decided a variable to test‚ say‚ hot vs cold water‚ you called this variable an “experimental variable” since it was the variable you were testing experimentally. In order to get meaningful data‚ you need to keep other things the same‚ for example the washing time had to be the same for both‚ you called the washing time a _Control_______ variable. Through the semester‚ you have used two bacteria that produce
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EFFECT OF USED OIL IN Trichoderma harzianum A Thesis Presented to the Faculty‚ Special Science Class Iloilo National High School La Paz‚ Iloilo City In the Partial Fulfillment in the subject Research III Sheree Joie Montomo Polonan January 2013 Chapter 1 Introduction of the Study Chapter 1 is divided into five parts: (1) Background of the Study‚ (2) Statement of the Problem and Hypothesis‚ (3) Significance of the Study‚ (4) Definition of Terms
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discovered by Lederberg and Tatum in 1946. In their experiment‚ they grew two strains of bacteria in separate vessels with rich medium and then together in one vessel containing the same medium. Then‚ they spread the three vessel contents onto medium agar plates and incubated them overnight at 37˚C. The only plate that showed cell growth was the plate containing the mixture of the two bacterial strains. The other two plates showed no growth. This experiment proved that in order for recombination to
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fermentation vs. respiration of microorganisms. One such medium‚ Thioglycollate Broth is useful for determining the oxygen relationship of a microorganism. The medium contains thioglycollic acid‚ cystine and agar‚ among other things. (Anaerobic Organisms Wikipedia).The thioglycollic acid and agar prevent oxygen from entering the entire medium. The medium used was a plate of TSA +glucose‚ one incubated anaerobically in a
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The first experiment involved agar cubes‚ each with a different surface area‚ to measure how surface area affects the rate of diffusion. In this experiment‚ the constants included 2% agar containing the pH indicator dye phenolphthalein‚ 1% phenolphthalein solution‚ 0.1M NaOH‚ 0.1M HCl‚ squares of hard‚ thin plastic (from disposable plates); unserrated knives; or scalpels from dissection kits‚ metric rulers‚ petri dishes or test tubes to hold the agar cubes. For the independent variable‚
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Historical synonym: Bacillus difficile. Morphology Gram-positive‚ straight rods‚ motility is variable‚ have a flagella. Spores are oval‚ sub terminal swelling the cell. Cultural characteristics Colonies on blood agar plates are circular‚ flat or low convex‚ opaque‚
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