technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile filter paper discs (using a disc dispenser)‚ which contain a known concentration of antibiotics. As soon as the antibiotic discs touch the agar‚ the antibiotic will begin to diffuse into the surrounding agar. During incubation the bacteria you inoculated onto the agar
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This is a quantitative test and is considered to be the gold-standard method for biofilm detection (13). Organisms isolated from fresh agar plates were inoculated in 10 mL of trypticase soy broth with 1% glucose. Broths were incubated at 370C for 24 hours. The cultures were then diluted 1:100 with fresh medium. Individual wells of sterile 96 well-flat bottom polystyrene tissue culture
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Pharmaceutical Microbiology CLASS CODE: MP202 SARA ASHOUR Signature ……………………………….. Date ……………………………….. Total Word Count 1492 I declare that‚ except where specifically indicated‚ all the work presented in this report is my own and I am the sole author of all parts. Toxic shock syndrome (TSS) is an uncommon but serious systemic infection which can occur in males and females of all ages. Symptoms include fever‚ hypotension
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quickly diffusion occurs is dependent on several factors‚ such as temperature‚ particle size‚ and the concentration difference on either side of the membrane. Testing the hypothesis relating to the effect of molecular weight to the diffusion rate‚ agar plate set-up was used to observe the diffusion of different substances. The methylene blue and potassium permanganate (KMnO4) was used and placed in the plate. Because the two substances have distinct colors‚ the measurement of the diffusion zone was
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was in order to test the many effects of diffusion and osmosis amongst four experiments. One such experiment was testing the effects of molecular weight on diffusion in relation to the use of Agar. The methods performed included the use of two acids‚ HCl and acetic acid. Both acids were placed into an Agar-filled dish and‚ over increments of 15 minutes‚ data collection was taken based off the diffusion rate and the diameter length of both the HCl and the Acetic Acid. The resulting factor was the
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Fisk‚ R.‚ John‚ J.‚ 2000. Services as theater. In: Swartz‚ T.‚ Iacobucci‚ D. (Eds.)‚ Handbook of Services Marketing and Management. Sage Publications‚ CA‚ pp. 21–35. Hollander‚ S.‚ 1960. The wheel of retailing. Journal of Marketing 25 (1)‚ 37–42. Hota‚ M.‚ 2008. Starbucks: brewing more than just coffee. European Case Clearing House (ECCC)‚ 508-025-1. Karolefski‚ J.‚ 2002. Conquering new grounds. BrandChannel‚ 11 February. (accessed 29.08.08.). Kiviat‚ B.‚ 2008. Wake up and sell the coffee. Time
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____________________ ABSTRACT The effect of molecular weight on the diffusion of substances were tested through the use of agar-water gel with three wells placed in a petri dish. Drops of three different substances namely: Potassium permanganate (KMnO4)‚ Potassium dichromate (K2Cr2O7) and Methylene Blue were placed in the wells of the agar-water gel in the petri dish. The diameter of the diffused substance was noted every 3 minutes for 30 minutes. The Potassium permanganate (KMnO4)
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a área disciplinar da “Dinâmica de Grupos” na tentativa de integrar todas essa terias sobre os fenómenos de grupos (Bales‚ 1985). Uma vez que as actividades em grupo exigem elevado grau de coordenação entre os elementos‚ principalmente em situações mais críticas‚ surgiu a necessidade de uma abordagem técnica e científica sobre o tema. Assim‚ o trabalho em grupo‚ ou o que vulgarmente se designa de trabalho em equipa‚ sendo certo que os termos apresentam diferenças‚ embora sejam muitas vezes utilizados
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STANDARD HOTEL: XX XX DATE: Mai-BS (Thailand) Company Limited Chamnan Phenjati‚ 25th Floor‚ 65/213; Pharam 9 Road‚ Huay Kwang‚ Bangkok 10310‚ Thailand KEYS OW CE EC EX FC FB FO GM P PM PRO S&M ALL = = = = = = = = = = = = = Owner Chief Engineer Executive Chef Executive Housekeeper Financial Controller Food and Beverage Manager Front office Manager General Manager Personnel Manager Purchasing Manager Public Relation Officer Sales Manager ALL CONCERNED Mai-BS (Thailand) Company Limited Chamnan
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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