002141-0012 Nuba Jackson IB Biology Microbiology IA How effective is Lysol in the reduction of bacterial growth compared to Pinesol in reduction of E. Coli growth in agar at room temperature? Background Information: Pinesol and Lysol are both common household disinfectants that make very big commercial claims; both claim to kill 99.9 percent of bacteria. Lysol contains
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fight‚ and would stop its growth. HA- If we add E. coli and B. subtilis to agar‚ and add Penicillin and Tetracycline to the agar‚ then the E. coli will grow more around the Penicillin and the B. subtilis will grow more around the Tetracycline‚ because E. coli is resistant to Penicillin and B. subtilis is resistant to Tetracycline. HO- If we add E. coli and B. subtilis to agar‚ and add Penicillin and Tetracycline to the agar‚ then the E. coli will grow more around the Tetracycline and the B. subtilis
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modelled using agar cubes soaked in the indicator phenolphthalein. The cubes will be cut into various sizes of 1cm to 3cm‚ then will be immersed into the acid‚ therefor the rate of diffusion can be measured by the decolouration that take place as the acid diffuses into the agar cube. Aim: To investigate how varying the surface area to volume ratio affects the rate of diffusion between three agar cubes. Hypothesis: The greater the surface area to volume ratio of the phenolphthalein agar cubes‚ the
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Read in your lab manual about the following agar mediums: Blood Agar (pg 168)‚ EMB Agar (pg 170)‚ Mannitol Salt Agar (MSA)(pg 172) )‚ MacConkey Agar (pg 174)‚ and PEA Agar (pg 176) to answer the following: 1. What does the blood agar select for? Blood agar allows distinction among bacteria based on their ability to lyse red blood cells (hemolytic activity). 2. What color is the blood agar? Blood red color. 3. What are the 3 types of blood agar results and how can you recognize them?
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“Super clean” and “Magic power”. Principal: Amalyse can catalyse the breakdown of starch into maltose. In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused to the star-agar. A clean zone will be formed around the wells when iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed
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te‚ rr*‚.ktn Bachelor’s Degree Programme (BDP) ASSIGNMENT (For July 2013 and January 2014 Sessions) Foundation Course in English FEG-02 fiort:nn frWw&ffiL;qLq*s/jr lfirf Ff {)pr.f .s *4ry:Ffl r uNtvERsrlnl School of Humanities Indira Gandhi National Open LTniversity Maidan Garhi‚ New Delhi-l10068 FOUNDATTON COURSE rN ENGLTSH (FEG-02) Programme: BDP/201 3-l 4 Course Code: FEG-02 Dear students. You are required to do one assignment for the Foundation Course in English-02
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suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior to use‚ melt and hold at 50c in a water bath 5 tubes of 9.0 ml trypticase soy (TS) broth 1 ml sterile pipettes Pipette aids Methods: 1. We began the experiment by marking the plates
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The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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In each tube a different kind of unknown bacteria was growing on the agar slant. Having the slanted agar allows for more surface area‚ therefore‚ more room for bacteria to grow. For each test there is a general set of rules to follow when transferring bacteria from one culture to another. Disinfect the table surface. Sterilize the transfer
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(in different amounts)‚ which can speed up the breakdown of proteins. As milk-agar plate is a milk protein‚ so when it is incubated with fruit juices containing proteases‚ the milk protein will be broken down and clear zones will appear around the wells containing different fruit juices. Thus‚ the higher the protease activity‚ the larger the diameter of the clear zones. Equation: Control: One well in the milk-agar plate is filled with distilled water to act as a control to show that the formation
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