culture on the EMB-lactose and PEA. Vancomycin wasn’t available to be used. We added our live culture to the EMB-lactose and PEA and added sterile beads to spread the bacterial cells all over the surfaces of the two agars and removed the beads and incubated the two agars. While the agars were incubating‚ we prepared our gel and loaded our respectful samples and ran the gel. After the gel finished running‚ we got a picture of our gel and recorded our observation for later
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Microbiology Module 02 Homework Assignment Use the information presented in this module along with additional outside research to answer the questions: 1) Compare and contrast prokaryotic and eukaryotic. a) Prokaryotes and eukaryotes are two types of cells that are very different but share some certain properties such as methods of reproduction‚ protein synthesis‚ an organized metabolism‚ response to stimuli‚ and plasma membranes. One significant difference is that prokaryotes are without a cell
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Materials • 30 petri dishes • Nutrient agar (a culture containing agar used to grow bacteria) • Sterile paper disks • Bacillus subtilis broth • Home-built incubator (a sealed box with a heat pad underneath) • Light box • Penicillin • Streptomycin • Tetracycline • Glutaraldehyde • 5 percent phenol alcohol • Fresh garlic extract Procedures 1. Divide petri dishes into six groups of five. 2. With adult supervision‚ melt the agar medium. 3. When liquid agar cools‚ pour an equal amount into each
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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Title: Screening of Cellulolytic Activity of Locally Isolated Thermophilic Fungi Title : Screening of Cellulolytic Activity of Locally Isolated Thermophilic Fungi Objective : To screen for thermophilic fungi as producer of fungal cellulase. Introduction One of the most important sources of carbon that is abundantly found on this planet is cellulose. While cellulase is the enzyme to degrade this carbon and it is a key enzyme in the bio refinery process of producing green chemicals
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THE CASE OF KATHPUTLI COLONY In 2007‚ the Delhi Development Authority (DDA) began planning for Delhi’s first in-situ slum rehabilitation project. It chose the Kathputli Colony (Kathputli)‚ a jhuggi jhopri (JJ) cluster tucked into West Delhi’s Shadipur region‚ as the site for this project. In 2009‚ Raheja—the private developers chosen to undertake the project—announced the construction
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well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow. This experiment allowed us to observe the process of bacterial transformation. I believe that only a small percentage of the cells will transform and the gfp plasmid will be most apparent in the agar plate containing both the plasmid and ampicillin.
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ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
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Gram-negative bacteria and fungal strains by measuring zone of inhibition. The antimicrobial activity was performed by Agar disc diffusion method at concentration level of 2.5‚ 5.0‚ 7.0‚ 10µg/ml respectively. Ampicillin (antibacterial)‚ Itraconazole (antifungal) as the standard drug at a concentration of 200µg/ml. LB Agar was used as the culture media for antibacterial and potassium dextrose agar was used as culture media for the antifungal activity. The results of the antimicrobial activity are shown in
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some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows: Lab day 1; today in lab we obtained the unknown mixed culture “041”and one brain-heart infusion agar (BHIA). The first step was the preparation of the medium‚ the bottom of the BHIA dish was labeled with the bacterium number‚ initials‚ and section; then divided into four quadrants. The second step‚ we used the septic technique to transfer a small
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