Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then the bacteria is non-hemolytic. For my bacteria no change is observed in the media therefore the bacteria is non-hemolytic and negative result. MacConkey Agar test
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Child Marriage in India If you have any questions regarding this information‚ feel free to email Juliana Shulman at: JShulman@uchicago.edu To be defined at length in a later section‚ child marriage is most simply‚ for our purposes‚ a marriage in which the wife is below the age of eighteen at the time of consummation. The practice of child marriage in rural India is deeply rooted in cultural values and grounded in social structures. And despite laws that prohibit child marriage‚ the practice
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of bacteria in a given cultured sample is too great to be counted directly. The samples will be serially diluted by a factor of 10 in sterile saline‚ to be repeated 7 times. A 10 microliter aliquot from each dilution will be placed on a 5% blood agar plate or a MacConkey plate‚ depending on the type of organisms plated. These plates are selected because of the ease of differentiation between organisms. The original vial and all diluted aliquots will then be refrigerated to restrict growth until
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technique). Each group will inoculate his/her own plate of Mueller-Hinton agar with an assigned culture. To that inoculated plate‚ you will then aseptically add sterile filter paper discs (using a disc dispenser)‚ which contain a known concentration of antibiotics. As soon as the antibiotic discs touch the agar‚ the antibiotic will begin to diffuse into the surrounding agar. During incubation the bacteria you inoculated onto the agar
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GO ERN~ E T OF ARU ACH 1 PRADESH OFFICE OF THE E TV CO ISSIO ’ER‚ leT A ~CH L A S BR-25 211 7108) q=j Date" 130 I d :a the 3 A...r;’Z010 To. ...-/’ Th Member Secreta Arunachal Prade h State Pollution Control Board Ita agar A nachalPradesh eport of the Public Hearin meeti 9 0 K Dibbin H dro Electric roject (120 MW) held a Community Hall‚ a r Town‚ West Kameng District‚ Arunachal Pradesh on 30 July 2010. OB.2009/Dibbin HEP/261 Su. R f: APSPC Dtlt-nagar‚ 2 ugu~t’ 2010
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This is a quantitative test and is considered to be the gold-standard method for biofilm detection (13). Organisms isolated from fresh agar plates were inoculated in 10 mL of trypticase soy broth with 1% glucose. Broths were incubated at 370C for 24 hours. The cultures were then diluted 1:100 with fresh medium. Individual wells of sterile 96 well-flat bottom polystyrene tissue culture
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Pharmaceutical Microbiology CLASS CODE: MP202 SARA ASHOUR Signature ……………………………….. Date ……………………………….. Total Word Count 1492 I declare that‚ except where specifically indicated‚ all the work presented in this report is my own and I am the sole author of all parts. Toxic shock syndrome (TSS) is an uncommon but serious systemic infection which can occur in males and females of all ages. Symptoms include fever‚ hypotension
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quickly diffusion occurs is dependent on several factors‚ such as temperature‚ particle size‚ and the concentration difference on either side of the membrane. Testing the hypothesis relating to the effect of molecular weight to the diffusion rate‚ agar plate set-up was used to observe the diffusion of different substances. The methylene blue and potassium permanganate (KMnO4) was used and placed in the plate. Because the two substances have distinct colors‚ the measurement of the diffusion zone was
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was in order to test the many effects of diffusion and osmosis amongst four experiments. One such experiment was testing the effects of molecular weight on diffusion in relation to the use of Agar. The methods performed included the use of two acids‚ HCl and acetic acid. Both acids were placed into an Agar-filled dish and‚ over increments of 15 minutes‚ data collection was taken based off the diffusion rate and the diameter length of both the HCl and the Acetic Acid. The resulting factor was the
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____________________ ABSTRACT The effect of molecular weight on the diffusion of substances were tested through the use of agar-water gel with three wells placed in a petri dish. Drops of three different substances namely: Potassium permanganate (KMnO4)‚ Potassium dichromate (K2Cr2O7) and Methylene Blue were placed in the wells of the agar-water gel in the petri dish. The diameter of the diffused substance was noted every 3 minutes for 30 minutes. The Potassium permanganate (KMnO4)
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