This was done using an inoculating needle and aseptically transferring the bacteria into a slant of Simmon’s citrate agar by stabbing the needle into the butt of the agar‚ then streaking it across the top of the agar as the needle was pulled out. The tube was then placed in the 37 degrees Celsius incubator for 48 hours‚ observed for a blue color‚ then placed back in the incubator for another 5 days and observed again
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Buffers‚ and pH‚ and Diffusion oh my The pH of a solution is the measure of the concentration of charged Hydrogen ions in that given solution. A solution with a pH lower than seven is considered to be acidic. A solution with a higher pH is a base. It is very important for organisms to maintain a stable pH. Biological molecules such as proteins function only at a certain pH level and any changes in pH can result in them not functioning properly. To maintain these constant pH levels‚ buffer solutions
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Isolation and Identification of Staphylococcus aureus and epidermidis Wendy Heck Bio 175: General Microbiology Fall 2012-11-21 Staphylococcus aureus is the most pathogenic for humans and Staphylococcus epidermidis is part of the normal flora and is of low pathogenicity. Staphylococcus epidermidis and Staphylococcus aureus are two medically important species of bacteria. A culture from the nose and throat was taken to perform whether or not Staphylococcus epidermidis or Staphylococcus aureus
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ascus. The spores germinate and form hyphae (Campbell‚ 64) Species of the genus Sordaria share a number of characteristics that are advantageous for genetic studies. They all have a short life cycle‚ usually 7-12 days‚ and are easy to grow on nutrient agar in dish cultures. All kinds of mutants are easily induced and readily obtainable with particular ascospore color mutants. These visual mutants aid in tetrad analysis‚ especially in analysis of intragenic recombination. ( Campbell 72) A common
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responses in sensitised people‚ causing respiratory diseases such as hayfever and asthma (Knox‚ Ladiges‚ Evans and Saints 2006). A variety of techniques can be used to identify the diversity and density of fungi within the Sydney Basin. The use of agar plate exposure allows microbial colonies to culture within a sterile environment. These colonies can be identified using dichotomous keys (School of Biological Sciences 2010) under compound and dissecting microscopes. This type of analysis is appropriate
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Human Development 13 CHAPTER conomic growth though important cannot be an end in itself. Higher standards of living as well as of development opportunities for all‚ stemming from the greater resources generated by economic growth‚ are the ultimate aim of development policy. This implies the need to bridge regional‚ social and economic disparities‚ as well as the empowerment of the poor and marginalized‚ especially women‚ to make the entire development process more inclusive. The draft Twelfth
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others. Two examples of selective medium are Mannitol Salt agar and Phenylethyl Alcohol. 2. What is a differential medium? What makes the medium differential? Name 2 examples (3 pts.) Differential medium is distinguishing microorganisms from one another based on growth characteristics. A medium is differential when you are able to visibly see the differences in growth patterns of organisms. Differential media include blood agar and Eosin Methylene Blue. Steps Used in Identifying an Unknown
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Antyodaya Anna Yojana (AAY) OBJECTIVE Antyodaya Anna yojana has been launched by the Hon’ble Prime Minister of India on the 25th December‚2000. This scheme reflects the commitment of the Government of India to ensure food security for all create a hunger free India in the next five years and to reform and improve the Public Distribution System so as to serve the poorest of the poor in rural and urban areas. It is for the poorest of poor that the Antyodya Anna Yojana has been
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Practical guide to detection and identification of Phytophthora Leaf blight A number of Phytophthora species cause leaf blight. These include: P. infestans on potato and tomato; P. palmivora on a large number of tropical fruit species including rubber‚ durian and macadamia; and P. colocasiae on taro. These blights on leaves are first seen as small flecks but within 3-5 days they expand to produce large lesions. Initially‚ infected tissue is water soaked but becomes necrotic (brown or black) in
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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