_____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform pore size than that of agar. It is also easily poured and does not denature the samples. In agarose gel electrophoresis‚ DNA or RNA fragments are separated or isolated according
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Making Agar Cushions 1. Start with wearing gloves and swabbing them with 70% ethanol to keep the materials that will be used in the experiment sterile. 2. Wipe the work station with Wescodyne and paper towels. It is important to keep the sterile materials such as slides in petri dish‚ Pasteur pipette in container and forceps covered as much as possible. 3. Place four to five filter papers in the petri dish and drip few drops of water on the strips‚ this will maintain moisture in the agar cushions
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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For this experiment you will need 39 agar plates‚ sterile cotton swabs‚ a concentrated amount of Escherichia coli (E. coli)‚ three different levels of antibiotic (Ampicillin: 1mg‚ 5mg‚ and 10mg)‚ a bunsen burner‚ metal tweezers‚ an incubator‚ 27 broths‚ and a ruler which should all be provided through your laboratory. For the first generation‚ you will be using 12 plates. Three of the plates will be labeled G1 control‚ three of the plates will be labeled G1/1 mg‚ three plates will be labeled G1/5
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Materials and Methods: Inoculation of the unknown was done on a nutrient slant agar The nutrient agar was then incubated at 27°C for one week. The agar appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain
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I. Product Background A. Introduction In this highly luxurious extravagant world‚ food and other beverages has always been one of the things which maintain the mainstream of life. Aside from being one of the basics for survival‚ gatherings are also made perfect by food preparation and stress is now often associated with food. This activities show that food intake nowadays is far different from the conventional times. Furthermore‚ this lavish lifestyle most of the people have results in an
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Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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Unknown #11: Citrobacter freundii Tryptic Soy Agar Test (TSA): TSA is a basic medium that is most similar to nutrient agar. The agar contains carbon‚ nitrogen‚ sodium chloride‚ and agar. This allows for the growth of a large variety of microorganisms to grow and ferment in the medium. Mannitol Salt Agar (MSA): MSA is a selective and differential medium that favors the growth of salt loving organisms. It is commonly used to distinguish the different species of Staphylococci. If an organism ferments
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LA SALLE GREEN HILLS Comparative Study Between the Bioplastic Properties of Agar-Agar (Gelidium amansii) and Potato (Solanum tuberosum) Starch Submitted by: Kyle Emmanuel A. David Rynno Gabriel Luis T. Garde Justin Carlo P. Gregorio Rufo Angelo M. Mauricio III Christian Michael A. Perreras II-B Submitted to: Miss Alvie Diaz Submitted on: January 30‚ 2012 ABSTRACT Bioplastic is a form of plastic derived from renewable biomass
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Hindi films are banned in Manipur since September‚ 2000‚ by separatist militant group Revolutionary Peoples Front which claims that Bollywood goes against Manipuri values. Priyanka Chopra is happy with all the critical praise coming her way for her performance as MC Mary Kom in the boxer’s biopic but the actress says it is “disheartening” that people from Manipur cannot see the movie. Kom too had expressed her sadness over her hometown missing its date with the big screen version of her life-story
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