bacteria Bacillus subtilis did not grow well to see if there was a clearing. After doing some research‚ it was shown that B. subtilis is lipase positive so there should have also been some clear sones like the Staphylococcus aureus (Watson). The DNAse agar could not have been analyzed because HCl was not available to precipitate the DNA to see if the bacterium had the enzyme DNase. If the test could have been analyzed‚ Staphylococcus aureus would be positive for DNase and E. coli would have been negative
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and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special
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in partial fulfilment of requirements in Biology 101.1 under Prof. Kimverly Hazel Coronel‚ 1st sem‚ 2013-2014 ABSTRACT The effect of molecular weight on the rate of diffusion was assessed using the agar-water gel test. The agar-water gel set up was composed of a six petri dish of agar-water gel containing two wells. Pinch of potassium permanganate (KMnO4) and methylene blue (C16H18N3SCl) were simultaneously introduced to each dish and measured the distance and its rate. Methylene blue‚ having
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Yellow | ColourlessMedia: Pink | ColourlessMedia: Yellow | Pink-RedMedia: Pink | ColourlessMedia: Pink | E. coli grown in EMB agar: As we can see from the table above‚ the streak of E. coli on a plate with EMB agar showed a metallic green sheen where E. coli was present. EMB stain is selective for gram-negative bacteria. It is made using 6:1 Eosin and Methylene Blue. EMB agar is a differential media and inhibits the growth of gram-positive bacteria while also using a colour indicator to tell the difference
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TCBS agar is a selective as well as differential culture medium used for isolation of Vibrio spp. The growth of intestinal flora other than Vibrio spp. is prevented by the high pH (8.5-9.5) of TCBS. TCBS agar consist off yeast extract‚ bacteriological peptone‚ sodium thiosulphate‚ sodium citrate‚ ox bile‚ sucrose‚ sodium chloride‚ ferric citrate‚ Bromothymol blue/ thymol blue and agar. Sodium thiosulphate‚ sodium citrate and ox bile are the components
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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of common household spices/herbs such as cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic‚ on the zone of inhibition in the Staphylococcus epidermis covered nutrient agar? Hypothesis: If 3 teaspoons of chopped cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic steep in half a cup of boiling water for 15 hours and then placed in nutrient agar covered with Staphylococcus epidermis (gram positive) bacteria for 24 hours (in an incubator)‚ garlic will have the strongest antimicrobial effect. This is because
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Bacteria will be grown in an agar broth containing .01%‚ .1%‚ .5% and 10% antibacterial soap. To fully understand the subject‚ information on everything necessary to conduct this experiment and comprehend the results was researched. Once the experiment is completed a conclusion will be able to be made as to whether or not bacteria can become resistant to antibacterial soaps The procedure for the experiment is as follows. Ecoli bacteria will be grown in agar broth with .01%‚ .1%‚ .5% and 10%
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using a glass tube and an agar-water gel. In glass tube test‚ two cotton plugs submerse with two different substances – hydrochloric acid (HCL) and ammonium hydroxide (NH4OH) on the other end of the tube. By their molecular weight‚ NH4OH has a small molecular weight that diffuses at a faster rate. While the formation of a white cloud was first occurred at the end of the tube containing HCL with a large molecular weight. In agar-water gel test‚ using agar plate and agar-water gel‚ 2 different substrate
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Escherichia coli Microbiology Lab NAS 185-14L Introduction In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is
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