Project 1: Antimicrobial Activity of Soil Isolates John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining
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Escherichia coli Microbiology Lab NAS 185-14L Introduction In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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of common household spices/herbs such as cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic‚ on the zone of inhibition in the Staphylococcus epidermis covered nutrient agar? Hypothesis: If 3 teaspoons of chopped cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic steep in half a cup of boiling water for 15 hours and then placed in nutrient agar covered with Staphylococcus epidermis (gram positive) bacteria for 24 hours (in an incubator)‚ garlic will have the strongest antimicrobial effect. This is because
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using a glass tube and an agar-water gel. In glass tube test‚ two cotton plugs submerse with two different substances – hydrochloric acid (HCL) and ammonium hydroxide (NH4OH) on the other end of the tube. By their molecular weight‚ NH4OH has a small molecular weight that diffuses at a faster rate. While the formation of a white cloud was first occurred at the end of the tube containing HCL with a large molecular weight. In agar-water gel test‚ using agar plate and agar-water gel‚ 2 different substrate
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The Book Thief critical essay Tallus Gibson Liesel acquires many books throughout living in Molching with the Hubberman’s but three that stick out are Mein Kampf‚ The stand over man and The Word Shaker. These three stuck out because they represent so much to her behind the actual words in the books. They are metaphors for Max and how important kind words are for combatting hateful ones. Just like Hitler said he would rule the world with his words‚ Liesel defends herself and max with the same
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Materials: 4 sterile cotton swab‚ sterile water in test tube‚ 4 agar plates and 2 blood agar plates‚ wax pencil and labels. Procedure: Appropriately label the cover of each plate as indicated in lab. 1. Determine 2 sampling sources‚ one from your body and one from the surrounding environment. 2. For the first agar plate‚ for sampling from air‚ remove the lids from the plate and allow it to sit uncovered for 15 minutes. 1. For second agar plate‚ open the “stick” end of the sterile cotton swabs to
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observable microbial colonies on the surface of the Agar solid‚ as to determine the presence of microbes in consumable products i.e. yoghurt and blue vein cheese. HYPOTHESIS: Microbial growth will be present in two of the three Agar plates (those containing the food product) due to the suspected presence of microbes‚ whilst the control Agar plate (containing no food products) will remain free of contamination and microbial growth. MATERIALS: - 3x Agar plates - 10g of berry yoghurt - 10g
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specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain‚ Catalase‚ Mannitol Salt Agar (MSA)‚ Blood Agar‚ Novobiocin‚ Coagulase‚ and DNAse (Alachi‚ 2007). Rebekah Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the
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of a wide variety of microorganisms whilst the agar function is the solid media onto which the bacteria can be isolated as independent colonies which are representatives of different bacterial species. Controlling microbial growth is necessary in numerous situations and is greatly significant in areas such as medicine. Growth of microorganisms is
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