An agar plate is a Petri dish that contains a combination of agar and nutrients that help microorganisms grow. The proper method of setting microorganisms on an agar plate is know as “streaking”. In order to streak‚ the microorganisms are placed on a sterile swab or metal wire‚ which is then dragged lightly against the agar solution‚ leaving behind the microorganisms. The amount of organisms is greatest at the beginning
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dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod‚ culture was taken from the tube and dragged across area 1 several time‚of the agar. The agar was then turned 90º‚ and the loop was flamed and cooled. Taking some culture from area 1‚ it was dragged over area two‚and the same steps were done for areas 3 and 4.Another technique used was spread-plate‚ where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod. The rod
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or magnesium environment will be significantly different than the rate of streaming in plain 2% agar and that calcium and magnesium will have an equal effect on cytoplasmic streaming in Physarum polycephalum because of their similar chemical properties. Methods: To set up P. polycephalum samples‚ 15 plates of agar were set up to culture the mold. After mixing 2M solutions of calcium and magnesium‚ agar was measured and mixed into the solutions and 5 plates of calcium solution‚ 5 plates of magnesium
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Introduction In my report I will discuss what I did as an experiment and what I hope for it to achieve. Firstly I carried out an experiment to assay the effectiveness that a range of disinfectants have on the growth of ecoli and whether or not it can prevent it from growing. From the experiment i should be able to see that some disinfectants have a greater effect than others do. From this I shall then draw a conclusion and evaluation on what was the most effective‚ and could there have been any
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count) of the total viable bacteria in the rice salad on a general non-selective agar using either the pour or the spread plate method. To confirm that the outbreak had been caused by any B. cereus present in the rice salad a selective media agar‚ such as mannitol egg yolk polymixin agar (MEYP/MYP)‚ should be used. Once B. cereus has been confirmed a further enumeration of the B. cereus should be performed on the MEYP/MYP agar selective media plate to show whether the amount of B. cereus present is within
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distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the smear was sequence covered with crystal violet
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of different concentration of protease. In this experiment‚ a plate of milk agar is needed for comparing the result of each sample. Circular wells were dug on the milk agar plates by the use of cork borer‚ and extracts could be put into the wells by dropper. One must note that milk-agar is white in color due to casein. If protease is present‚ casein will be digested by the enzyme giving a clear zone in the milk-agar plate. The size of the clear zone can help us to determine whether fruits differ
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Question: What antibiotics work best in preventing E. coli k12 from growing; amoxicillin‚ ampicillin‚ or ciprofloxacin? Hypothesis: If ciprofloxacin‚ an antibiotic‚ is added to petri dishes covered in live bacteria and left to sit for four to six days in an incubator at 37 degrees Celsius‚ then the petri dishes containing ciprofloxacin will have the largest zone of inhibition out of all of the other antibiotics tested because research shows that ciprofloxacin in one of the strongest antibiotic currently
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urease. Casein hydrolysis tests for organisms capable of hydrolyzing casein via the casease. Materials and Methods Bile Esculin Hydrolysis The organisms Lactococcus lactis and Enterococcus faecalis were spot-inoculated on a bile esculin agar plate. The bile esculin agar plate is a both selective and differential medium contains primarily esculin. The plate was then inverted and incubated at 37 oC for 24 hours. Bile salts‚ the selective agent‚ can allow only Enterococcus and group d streptococcus to hydrolyse
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the Gram stain‚ and light microscope identify at least 2 Prokaryotes (bacteria) found in the water samples that are isolated on the MacConkey agar plates and the nutrient agar plate. Using the Identification Lab manual‚ identify at least 2 Eukaryotes (fungus) found in the soil sample that are isolated on the Potato dextrose agar plate and the nutrient agar plate. 3. In an agricultural context‚ research bacteria and fungus and their importance to Earth. 4. A high quality‚ 3+ resource
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