early days of microbiology in the 19th century‚ culture on agar plates has been a central technique for the study of bacteria. This practical is designed to introduce students to the basic techniques required to manipulate bacteria. Students will gain experience with the streak plate procedure‚ used to isolate pure colonies of bacteria‚ and viable plate count methods. The latter involves serial dilution and spread plating of bacteria on agar plates. Materials required per pair • One 10 ml liquid culture
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Title Antimicrobial properties in different type of plants. Introduction A substance that kills or prevents the growth of microorganisms for example bacteria‚ fungi or protozoans is called an antimicrobial. This substance has 2 major roles which are to either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic). Disinfectants are antimicrobial substances used on non-living objects outside the body. This substance included antibiotics‚ antifungals‚ antiprotozoals and
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is believed that higher temperatures will assist in the growth rate in the agar plates. Therefore it is believed that the agar plates placed in full light will produce more bacteria. Due to the type of light used for the full light part of the experiment there will be higher temperatures and therefore grow better than the no light and day light plates which are at lower temperatures. Equipment and Materials: 1 6 agar plates 2 E. coli and S. albus bacteria 3 Light 4 Day light 5 Cupboard
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Advances in Fish Microbiology and Pathology (FIS 508) Dr. Akinyemi‚ A. A. Aquaculture and Fisheries Management University of Agriculture‚ Abeokuta‚ NIGERIA. Microorganisms • Microorganisms is the existence of every minute living organisms or they are living features that can be seen with the aid of microscope‚ microscope‚ most of them are normally singlecelled while some may exist in multicellular forms. • These microorganism‚ though minute and microscopic‚ are a very powerful
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alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable‚ 1 Pencil‚ marking‚ 11 Petri dish‚ 60 mm‚ 2 Candles (flame source)‚ 1 Thermometer-in-cardboard-tube‚6 Test Tube(6)‚ 16 x 125 mm in Bubble Bag‚ 1 Test tube holder‚ 1 Test-tube-rack-6x21-mm‚ 1 Pipet Graduated Small (5 mL)‚ 1 Baker’s Yeast Packet – Saccharomyces cerevisiae‚ 1 Agar‚ MRS - 18 mL in Glass Tube‚ 4 Agar‚ Nutrient - 18 mL in Glass Tube‚ 1 Broth
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Apparatus: • Agar Powder • Distilled Water • McCartney Bottles • Brassica Seeds • Scissors Diagram of Apparatus: Method: • Place some Rapid cycling Brassica seeds onto a damp sponge placed in a plastic tray. Cover with cling film and place in a warm‚ light place to germinate. • When the seeds have germinated‚ they are ready to culture. • Measure out 2.5 g of agar powder and add to 250 cm3 of distilled water. Heat and stir gently until the agar dissolves
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cotton swabs are first made damp using sterilized water. The swabs are rubbed in the following 5 places and then rolled and rubbed in the petri dishes: A: Rub the swab on the pass-code for the main entrance of the school and then roll it over the agar in petri dish A. B: Rub the swab on the door handle in room 3.1 and then
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the centre of the cells further away from the surface. Therefore‚ when dipped in sodium hydroxide‚ the larger cells will not be dyed pink all the way through like the smaller cells after the ten minutes. Materials: 1x1x1 cm agar cube 2x2x2 cm agar cube 3x3x3 cm agar cube 100 ml of sodium hydroxide Goggles Petri dish Scissors Timing device Tweezers Beaker Graduated cylinder Procedure: Refer to ’Nelson Science Perspectives 10’ pages 38-39‚ section 2.4; ’What Limits Cell Size?’.
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(Salivary amylase) against temperature” aims to know and observe the enzyme activity of the human saliva. The research only included the use of starch-agar as the medium to observe enzyme activity during the experiment. Five starch-agar plates were prepared and each were labeled 1‚ 2‚ 3‚ 4 and 5 respectively. One mL of saliva were placed in each starch-agar plate which was holed then incubated for 24 hours. The plate labeled 1 was stored with 0ºC. The plate numbered 2 was stored at 15ºC‚ plate 3 at
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A2 Practical Investigations Title: The effect of Temperature on the growth of Aspergillus oryzae Develop a Hypothesis This particular investigation is to discover how a range of temperatures effects the growth rate of the fungi Aspergillus oryzae. Most fungi’s tend to survive within the temperature range of 5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot
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