Day 1 Throughout the semester‚ I have learned multiple techniques for identifying bacteria. Learning how to gram stain‚ use specific media such as MacConkey agar‚ and test antibiotics to see which antibiotic would react best against a specific organism. All these techniques helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive
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cells growing on soft agar burst from the viral infection and appears like a hole in the agar. Each plaque is created by the progeny of an individual phage and can thus be counted to determine the number of phage particles in a sample. The purpose of this lab is to employ a plaque assay method to determine the number of infected phage particles in the given sample. Method & Materials: * (6) BHI plates at 37˚C * (5) tubes of 9.0mL TSB broth * (6) tubes of soft agar in a 50˚C water bath
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cold CaCl2 solution. Label one tube with your initials and a (+) and the other tube with your initials and a (-). 2. Transfer 2-4 large colonies using a sterile plastic loop to each microcentrifuge tube and completely resuspend. Do not transfer any agar. Put the tip of the loop into the CaCl2 solution and spin until there is not any cells on the loop. 3. Close each of the tubes and put them in ice. 4. Ask your teacher to
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MATRIC MUSIC 2014 SET WORK Franz Schubert Der Erlkönig The Romantic Era The romantic period in music extended from about 1820 to 1900. Among the most significant musicians were Franz Schubert‚ Robert Schumann‚ Clara Wieck Schumann‚ Frederic Chopin‚ Franz Liszt‚ Felix Mendelssohn‚ Hector Berlioz‚ Peter Ilyich Tchaikovsky‚ Antonin Dvorak‚ Johannes Brahms‚ Giuseppe Verdi‚ Giacomo Puccini‚ Richard Wagner and
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Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
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aane par sab logo ne ghee ke diye jalaye Bhains ke aage been bajana Aankho mein dhool jhokhna Mitti mein mila dena Naak mein dum aa jana Din mein tare dikhna Ghutne tek dena- haar man lena Eid ka chand hona- kabhi kabhoi hi dikhai dena Aasman sir par uthana- bahut shorgul karna Tas se mas ne hona- apne zid par ade rehna Tang adana – bekar dakhal dena Andhe ki lathi- 1 matr sahara Aakash pataal 1 kar dena Dal mein kuc kala hona- sandeh ki baat hona’ Daat khatte karna- buri tarah haraana
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Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies that grew on the MSA were used to inoculate Gram-positive tests‚ where as MacConkey Agar colonies were used to inoculate Gram-negative tests. Once the colonies were isolated and the appropriate Gram-negative and Gram-positive tests were conducted‚ the identification of both unknown organism were fairly easy. The results from the
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disk‚ impregnated with the compound to be tested‚ is placed on the surface of the agar. The compound diffuses out from the filter paper into the agar. The concentration of the compound will be higher next to the disk‚ and will decrease gradually as distance from the disk increases. If the compound is effective against bacteria at a certain concentration‚ no colonies will grow wherever the concentration in the agar is greater than or equal to that effective concentration. This region is called the
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purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials : 1) Blood agar plate . 2) Mannitol Salt agar (MSA) plate. 3) DNase agar plate . 4) Novobiocin disc . 5) Inoculating loop. 6) flame ( Bunsen burner) . 7) 1N hydrochloric acid (HCl) . 8) Two slides . 9) Plasma tube. 10) 3% Hydrogen Peroxide (H2O2) . 11) One unknown plate . 12) Crystal
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behaviors of different cellular slime molds were analyzed under different conditions. They found that in D. disciodeum thrived equally on nutrient soil and a non-nutrient agar dishes (Bonner & Lamont‚ 2005). Similarly to the experiment preformed by Bonner and Lamont‚ we evaluated growth patterns of D. disciodeum on non-nutrient agar dishes. Another study conducted by Fisher‚ found that certain genetic factors increase the movement and development of D. disciodeum amoebae within a temperature gradient
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