the way physicians’ control/treat infectious disease within our bodies. In this lab‚ chemotherapeutic agents were evaluated by the disk-diffusion method. Chemotherapeutic agents are placed on the surface of a Petri plate containing Mueller-Hinton agar‚ which allows the agents to diffuse freely‚ and a growth medium is incubated over its’ surface. During incubation‚ the chemotherapeutic agents move from levels of high concentration‚ to low levels of concentration. To be effective‚ the agent must inhibit
Premium Management Marketing German language
Experimental Methods In this lab‚ we use the procedure from Chemically Defined‚ Complex‚ Selective‚ and Differential Media. We divide the agar plates that have the unknown in third‚ write our initials‚ and date on the bottom of the agar plate. We also divide the agar plates into quadrants‚ label each organism in their quadrant‚ write our initials‚ and date on the bottom of the agar plates. We flame the loop and let it cool for a minute‚ we remove the cap of each culture tube of organisms with our pinky while
Premium Water Oxygen Bacteria
ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
Premium Bacteria Gram staining Microbiology
INTRODUCTION Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria‚ yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method
Free Bacteria Agar plate Growth medium
satisfactory method employed to evaluate the effectiveness of antiseptic agents against selected test organism. In this method‚ sterile filter paper disk is impregnated with an antiseotic and placed on fresh heavily inoculated agar plate of a test organism. Following incubation‚ the agar plate is looked for a zone of inhibition. The presence of a clear zone of inhibition surrounding the disk is indicative of inhibitory (antimicrobial) activity against the organism. This experiment deal with the evaluation
Premium Antiseptic Bacteria Microbiology
Experiment No. 03 Date: Name of the experiment: Aerobic plate count (APC) and total coliform count of supplied yogurt sample. Purpose: To determine the aerobic plate count and total coliform count of the supplied yogurt sample. Principle: Yogurt squeezed or extracted from milks are more or less acidic‚ depending on the product‚ the pH generally ranges from about 2.4-4.2 and contain sugar (4.7g)‚ fat
Premium Bacteria Acetic acid Carboxylic acid
effect of size on the effectiveness of diffusion Aim: To use agar blocks infused with 0.1 Molar sodium hydroxide (NaOH) and phenolphthalein to investigate the relationship between shape and surface area: volume ratio on the effectiveness of diffusion. Hypothesis: That for a cube of agar‚ the time taken for complete colourisation due to diffusion of HCl is directly proportional to the cubes volume. Materials: |A block of agar (10cm x 5cm x 3cm) with 0.1M NaOH and |1x 250mL beaker
Premium Volume Sodium hydroxide Orders of magnitude
microscope to identify its unknown characteristic. There were other methods utilized in lab as well: the Mannitol Salt and Eosin Methylene Blue Agar and the tryptic soy broth experiments. Oxygen reaction (aerobic vs. anaerobic)‚ glucose fermentation‚ oxidase reaction‚ the catalase test‚ urea hydrolysis‚ nitrate reduction experimentation‚ Kligler’s Iron Agar‚ the SIM medium test and lastly the IMViC series of tests. All the biochemical tests were carried out in properly supervised manner to compare
Premium Bacteria Escherichia coli Microbiology
uses of bacteria. Throughout the investigation a series of experiments will be conducted to determine if metals such as nickel and aluminium have a resistance to E.coli K12 over a sequence of generations. To complete the investigation four Macconkey agar plates were inoculated with E.coli k12 and four paper discs of aluminium and nickel that were soaked in metal salts were placed into the plates to see the resistance that would occur over. Once resistance had occurred‚ the zone of inhibition was scraped
Free Bacteria Antibiotic resistance Escherichia coli
RESEARCH PAPER ON BIOLOGICAL ACTIVITY OF ACTINOMYCETES ................ ABSTRACT: The microorganisms are ubiquitous in nature. They are found in probably every environmental condition present on earth. Actinomycetes were originally considered to be an intermediate group between bacteria and fungi but now are recognized as prokaryotic organisms with high G+C (>55%) content in their DNA. Our project aim is to evaluate some biological activities of Actinomycetes like:
Premium Enzyme Petroleum Bacteria