Contributors to Bacteriology In an unassuming way‚. they moved agar from the kitchen to the lab‚ revolutionizing bacteriology WOLFGANG HESSE T RANSLATED BY D IETER H. M. GR~SCHEL Walther Hesse was a well-known community health physician in the Kingdom of Saxony‚ a student of Max von Pettenkofer‚ the father of hygiene‚ and of Robert Koch‚ the father of medical microbiology. His American wife‚ Fanny Angelina‚ introduced agar-agar to the new science of microbiology. The Hesse Family Walther
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the glass tube test and the agar-water gel test. In the glass tube set-up‚ two cotton plugs soaked in two different substances (HCl and NH4OH) were inserted into the two ends of the glass tube. The substance with the lighter molecular weight value (NH 4OH‚ M = 35.0459 g/mole) diffused at a faster rate (dAve = 25.8cm)‚ resulting in the formation of a white ring around the glass closer to the side of the heavier substance (HCl‚ M = 36.4611 g/mole; dAve = 10.8 cm). The agar-water gel set up was composed
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various petri dishes containing agar‚ E Coli and the slime mold Dg‚ and the pH level is changed‚ then the petri dishes containing pH 4 (potassium acid phthalate) and pH 5 (sodium and potassium phosphate) will grow fruiting bodies more rapidly than pH 7 (potassium phosphate) and pH 9 (potassium phosphate and sodium borate)‚ since slime molds have a tendency to move away from ammonia‚ they probably do not prefer basic solutions. METHOD Materials: 4 petri dishes with agar and E Coli Dg pH 4 – potassium
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Leisel to read. By doing this he is releasing her mind to a world where she can understand the true meaning of the words. Therefore Hans unlocks Leisel’s mind to the true horrific messages that come across in Hitler’s speeches and Hitler’s writings in Mein Kampf. By teaching Leisel to read‚ Hans also frees her mind and distracts her from feeling guilt for her brother’s death‚ he helps Leisel to learn that she could have done nothing to save her brother‚ death took him and it was his destiny to die on
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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in a color change of the medium from green to blue. Materials needed include simmons citrate agar slant tube‚ and a pure culture of bacteria. The starch hydrolysis test is used to determine the ability to hydrolyze starch. Bacteria use amylase‚ an extracellular enzyme to hydrolyze bonds linking the glucose subunits. Starch is detected by adding iodine to the medium. If the bacteria growing on the starch agar produce amylase‚ no color change will be seen around the bacterial growth because all of the
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was place on a rack then the stain were apply. B. From agar cultures. 1. A drop of water was place in the center of the glass slide. 2. With sterilized inoculating loop to obtain a minute of bacterial culture from the agar cultures 3. It then mix with the drop of water and then proceeds as broth cultures. SIMPLE STAINING TECHNIQUES Material: 1. 24 hours broth cultures of a. E. coli b. Staph. aureus 2. 24 hours nutrient agar slants of a. Bacillus subtilis b. Pseudomonas aeruginosa
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In this lab you will test the idea that microbes are ubiquitous or present everywhere at all times. Materials One Triptic Soy Agar (TSA) plate per student One sterile swab Procedure 1. Decide on an area to test for the ubiquity of microbes. List your area on the white board in front of the classroom. 2. Obtain one TSA plate and label it on the bottom (side with the agar) with your name‚ class section and the surface you will sample. 3. Obtain one sterile swab. 4. To obtain a sample‚ roll the sterile
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ratio is considered to be the most significant factor in triggering a cell to divide‚ and therefore‚ determining cell size. OBJECTIVES • Determine the extent and rate of diffusion into three different size agar cubes. • Calculate the surface area to volume ratio for each agar
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Your Full Name: UMUC Biology 102/103 Lab 1: Introduction to Science INSTRUCTIONS: On your own and without assistance‚ complete this Lab 1 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus). To conduct your laboratory exercises‚ use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering
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