Making Agar Cushions 1. Start with wearing gloves and swabbing them with 70% ethanol to keep the materials that will be used in the experiment sterile. 2. Wipe the work station with Wescodyne and paper towels. It is important to keep the sterile materials such as slides in petri dish‚ Pasteur pipette in container and forceps covered as much as possible. 3. Place four to five filter papers in the petri dish and drip few drops of water on the strips‚ this will maintain moisture in the agar cushions
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4.5 DISCUSSION The bioluminescent bacteria grow well and produce good glowing in the SWC agar media compared to the LA agar media. In LA agar media‚ the production of light was very deem. It also took much time to solidify and the agar media was too soft and forms hole‚ therefore good streaking couldn’t be done. There might be error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing
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For this experiment you will need 39 agar plates‚ sterile cotton swabs‚ a concentrated amount of Escherichia coli (E. coli)‚ three different levels of antibiotic (Ampicillin: 1mg‚ 5mg‚ and 10mg)‚ a bunsen burner‚ metal tweezers‚ an incubator‚ 27 broths‚ and a ruler which should all be provided through your laboratory. For the first generation‚ you will be using 12 plates. Three of the plates will be labeled G1 control‚ three of the plates will be labeled G1/1 mg‚ three plates will be labeled G1/5
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Although it is thought of as having been ’written’ by Hitler‚ Mein Kampf is not a book in the usual sense. Hitler never actually sat down and pecked at a typewriter or wrote longhand‚ but instead dictated it to Rudolph Hess while pacing around his prison cell in 1923-24 and later at an inn at Berchtesgaden. Reading Mein Kampf is like listening to Hitler speak at length about his youth‚ early days in the Nazi Party‚ future plans for Germany‚ and ideas on politics and race. The original
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Materials and Methods: Inoculation of the unknown was done on a nutrient slant agar The nutrient agar was then incubated at 27°C for one week. The agar appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain
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I. Product Background A. Introduction In this highly luxurious extravagant world‚ food and other beverages has always been one of the things which maintain the mainstream of life. Aside from being one of the basics for survival‚ gatherings are also made perfect by food preparation and stress is now often associated with food. This activities show that food intake nowadays is far different from the conventional times. Furthermore‚ this lavish lifestyle most of the people have results in an
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Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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Should “Mein Kampf” be banned? At some point and time everyone has had a moment when they reflect back to a certain situation where they would have the deposition of reversing time to repair the situation. Such as the time‚ when you had the most embarrassing moment of your life.So‚ now you want to reverse time and prevent yourself from doing that terrible mistake that wasn’t intended to occur. This is similar to what happened with the holocaust‚ it was something that many people look back and
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Topic – Reliance – Kar Lo Duniya Mutthi Mein e. The specific aspects that you want to study or the focus of the study : Innovation f. The approach to be used for doing the study: Kar Lo Duniya Mutthi Mein Name of the Participant: Sneha Nayak Current Organisation: Ness Technologies Pvt Ltd. Designation: Lead Business Analyst. No. of years of Exp : 6+ Name of the Term Paper : Reliance Industries limited Topic: Kar Lo Duniya Mutthi Mein I have chosen this company to submit
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Hitlerian Nationalism What was the nature of the nationalism used by Hitler in Mein Kampf? Where did it’s focus lie? To what extent was this nationalism merely a tool of Hitler’s ambition and to what extent did he really believe in it? Before being able to study the extent to which Hitler employed nationalism as a tool in his policies evident in his seminal work Mein Kampf (My Struggle/Battle) we must first clarify what we understand to be his definition of nationalism. Nationalism can cover
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