count) of the total viable bacteria in the rice salad on a general non-selective agar using either the pour or the spread plate method. To confirm that the outbreak had been caused by any B. cereus present in the rice salad a selective media agar‚ such as mannitol egg yolk polymixin agar (MEYP/MYP)‚ should be used. Once B. cereus has been confirmed a further enumeration of the B. cereus should be performed on the MEYP/MYP agar selective media plate to show whether the amount of B. cereus present is within
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since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide. Fixing = passing the smear through the flame of the laboratory burner three times in rapid succession
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suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior to use‚ melt and hold at 50c in a water bath 5 tubes of 9.0 ml trypticase soy (TS) broth 1 ml sterile pipettes Pipette aids Methods: 1. We began the experiment by marking the plates
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Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
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used to test antimicrobial activity against disease causing bacteria Staphylococcuaureus. Extracts of varying concentrations of Azadirachtaindica fruit pulp and leaf extract were prepared using Phosphate Buffer and tested against test organisms using agar diffusion method. Oxfloxacinof same varying concentrations were used to compare the effect of antimicrobial activity of fruit pulp and leaf extract. Keywords: Azadirachtaindica‚ Antimicrobial activity‚ Staphylococcus aureus. I. INTRODUCTION Azadirachtaindica
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were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings herein and reflect the results of microscopic observations as well as the differential tests results on various agars and broth cultures. Although all tests were not conclusive‚ the unknown organism labeled Unknown #11 was found to be a member of the family Enterobacteriacea and Genus Serratia marcescens. INTRODUCTION The field of Microbiology is the
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_____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more uniform pore size than that of agar. It is also easily poured and does not denature the samples. In agarose gel electrophoresis‚ DNA or RNA fragments are separated or isolated according
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microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol salt agar‚ blood agar and MacConkey agar plates which must be used based on the components of the bacteria. The catalase test will be used to understand the difference in facultative anaerobic and groam positive from aero tolerant anaerobes. The coagulase test converts fibrogen
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a given sample. It enumerates the number of actual live‚ viable cells in the sample that form colonies on a suitable agar medium. As the optimum medium and conditions varies for one sample to another‚ the colony count methods provide an estimate of the number of viable cells according to the medium employed‚ time and temperature of incubation. Each colony that appears on the agar plate arising either from a clump of cells or from a single cell is referred as a colony forming unit (CFU). The sample
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CHAPTER ONE 1.0 INTRODUCTION Natural rubber is produced by over 2000 plants species and its main constituent is poly (cis-I‚4-isoprene).a highly unsaturated hydrocarbon. Since 1914 there have been efforts to investigate microbial rubber degradation: However‚ only recently have the first proteins involved in this process have been identified and characterized and have the corresponding genes cloned. Analysis of the degradation product of natural rubber and synthetic rubbers isolated from various
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