completed on an agar plate but on one a much larger scale and where techniques can be perfected before the use of agar plates and specimens are used. Because this experiment uses materials that represent those used in the streaking on agar plates the ability to simulate the events that occur when streaking is similar and allows for visualization instantly. In this experiment materials will be gathered that are representative of the tools need to complete the actual experiment of agar plate streaking
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for antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚ was spread on the same plate and reincubated
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on bacterial growth. By observing the effects of 5 different inhibitors including alcohol‚ bleach‚ soap solution and distilled water‚ it was determined what antiseptic or disinfectant was able to best inhibit this kind of bacterial growth. Nutrient Agar was poured into a Petri dish with four quadrants and then a pipette was used to place bacterial culture on top. Using forceps‚ a filter disc was dipped into each inhibitor and then into a separate quadrant of the Petri dish. The lid of the Petri dish
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LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO.- 10800037 ROLL NO- RB1R07B02 B.TECH BIOTECH.(8th sem.)
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INTRODUCTION Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria‚ yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method
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TSI test because there is no pink color for Urea test and no black color on the bottom of the tube in TSI test. Then‚ follow three tests results are all positive. That is deep blue color for citrate test‚ clear zone surrounding growth for skim milk agar
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there are structures called ascospores. It is these structures‚ ascospores‚ where genetic variation that arises from crossing over is easily seen (Davidson). The organism Sordaria Fimicola is a good example of this process because it is easy to grow on agar plates and because they are easy to be seen when looked at through a microscope (Davidson). There are three strands of Sordaria Fimicola used in this experiment; all were retrieved from an area known as the Evolution Canyon. The Evolution Canyon has
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same control on both sides of the knob and disinfect each side with different disinfectants. There were intervals of 4 minutes‚ in which we let the bacteria sit‚ and swabbed the bacteria off. Immediately after‚ we inserted the sample on a nutrient agar dish. Finally‚ we incubated the samples for approximately 2
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moved from one organism to another with the use of pGlo plasmids. It was hypothesized that if bacteria that were transformed with +pGlo plasmids are given the gene for GFP‚ then transformed cell colonies will be located on the LB/amp/ara and LB/amp agar plates. Cells that have been transformed with +pGlo plasmids have the ability to grow in ampicillin plates‚ and the arabinose sugar allows the colonies to be visibly fluorescent under ultraviolet light. The GFP is able to resist ampicillin because
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Abstract Introduction An antimicrobial is a substance that kills or inhibits the growth of microorganisms such as bacteria‚ fungi‚ or protozoans (Antimicrobial). Antimicrobial drugs either kill microbes or prevent the growth of microbes. Disinfectants are antimicrobial substances used on non-living objects or outside the body. Ginger Figure 1 : Ginger (Studies Reveal Ginger Lowers Colon Cancer Risk) Ginger is commonly used around the world and has been employed in the treatment‚ cure‚ and
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