for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars‚ various other tests need to be done to differentiate and confirm their identity. RESULTS Bacteriology – When grown on MacConkey agar‚ A had abundant growth of pink punctiform colonies that are circular and moist. Microbes A are gram-negative cocci. B had moderate growth of yellow punctiform colonies that are circular
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David Kennedy Bio 210 Lab Report 1 10/11/13 Microbial Growth Background Information: This lab was conducted in order to understand basic differences among differential and selective media‚ while recognizing how each media is used to isolate and identify microorganisms (Wistreich‚ 2003). The first microorganism analyzed was Staphylococcus epidermidis. This organism is gram-positive‚ single celled‚ arranged in grape-like clusters‚ and cocci in shape (Bukhari‚ 2004). S. epidermidis is approximately
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groups then placed in agar plates simulating different environments: the bacteria lacking the pGLO plasmid was subjected to an environment solely contaiing nutrients and another containing nutrients and ampicillin; the bacteria containing pGLO was subjected to an environment containing solely nutrients and one containing nutrients‚ ampicillin‚ and the sugar arabinose. After being allowed to grow in their respective environments‚ the following agar plates grew E. Coli colonies: agar containing nutrients
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Introduction: Microbes‚ also called microorganisms‚ are minutes living things that individually are usually too small to be seen with the unaided eye. The group includes bacteria‚ fungi (yeast and molds)‚ protozoa and microscopic algae. It also includes viruses‚ those noncellular entities sometimes regarded as straddling the border between life and nonlife. People tend to related these microbes only with major disease such as AIDS‚ uncomfortable infections‚ or such common inconveniences as spoiled
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fight‚ and would stop its growth. HA- If we add E. coli and B. subtilis to agar‚ and add Penicillin and Tetracycline to the agar‚ then the E. coli will grow more around the Penicillin and the B. subtilis will grow more around the Tetracycline‚ because E. coli is resistant to Penicillin and B. subtilis is resistant to Tetracycline. HO- If we add E. coli and B. subtilis to agar‚ and add Penicillin and Tetracycline to the agar‚ then the E. coli will grow more around the Tetracycline and the B. subtilis
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modelled using agar cubes soaked in the indicator phenolphthalein. The cubes will be cut into various sizes of 1cm to 3cm‚ then will be immersed into the acid‚ therefor the rate of diffusion can be measured by the decolouration that take place as the acid diffuses into the agar cube. Aim: To investigate how varying the surface area to volume ratio affects the rate of diffusion between three agar cubes. Hypothesis: The greater the surface area to volume ratio of the phenolphthalein agar cubes‚ the
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“Super clean” and “Magic power”. Principal: Amalyse can catalyse the breakdown of starch into maltose. In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused to the star-agar. A clean zone will be formed around the wells when iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed
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The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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In each tube a different kind of unknown bacteria was growing on the agar slant. Having the slanted agar allows for more surface area‚ therefore‚ more room for bacteria to grow. For each test there is a general set of rules to follow when transferring bacteria from one culture to another. Disinfect the table surface. Sterilize the transfer
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(in different amounts)‚ which can speed up the breakdown of proteins. As milk-agar plate is a milk protein‚ so when it is incubated with fruit juices containing proteases‚ the milk protein will be broken down and clear zones will appear around the wells containing different fruit juices. Thus‚ the higher the protease activity‚ the larger the diameter of the clear zones. Equation: Control: One well in the milk-agar plate is filled with distilled water to act as a control to show that the formation
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