patients such as those suffering from cystic fibrosis or immunocompromised individuals. MIC methods are varies such as disc tests that give qualitative result‚ dilution tests which testing the ability of microorganisms to produce visible growth on several agar plates. It containing dilutions of the culture and antimicrobial agent.
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known as "red seaweed." The specific chemical that we are interested in is agar‚ which appears in red seaweed in abundance. When you hear Cereplast and other companies talk about developing bioplastic made from seaweed‚ they really mean that they will be using the chemical agar‚ which is extracted from the seaweed. Fortunately (or unfortunately?) this project won’t have you traipsing out to the ocean to collect seaweed. Agar is used as a food additive in confectionaries‚ desserts‚ beverages‚ icecream
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stain. * Observe the slides under microscope and record results. BIOCHEMICAL TESTS SUSCEPTIVITY TESTING Materials * MHA agar * Organism grown in Broth * Swabbing stick * Principle Procedure TSI (TRIPLE SUGAR IRON TEST) Materials * TSI agar * Straight wire loop * Cultured colony of organism * Purity plate Principle TSI agar detects fermentation of glucose 0.1%‚ lactose 1% and sucrose 1%‚ production of H2S‚ and gas formation. The medium contains ferric
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analysis. Most frequently‚ the identification of a specific organism must be accompanied by a sensitivity study. Also due to the changing patterns of resistance in other bacteria to antibacterial agents‚ sensitivity studies are essential. Clinically‚ the agar diffusion method is commonly used. The organism can be reported as being sensitive‚ intermediate or resistant to an antibiotic. Sensitivity depends on the growth characteristics of the organism and diffusion characteristics of the antibiotic. Some
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bacteria Bacillus subtilis did not grow well to see if there was a clearing. After doing some research‚ it was shown that B. subtilis is lipase positive so there should have also been some clear sones like the Staphylococcus aureus (Watson). The DNAse agar could not have been analyzed because HCl was not available to precipitate the DNA to see if the bacterium had the enzyme DNase. If the test could have been analyzed‚ Staphylococcus aureus would be positive for DNase and E. coli would have been negative
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TCBS agar is a selective as well as differential culture medium used for isolation of Vibrio spp. The growth of intestinal flora other than Vibrio spp. is prevented by the high pH (8.5-9.5) of TCBS. TCBS agar consist off yeast extract‚ bacteriological peptone‚ sodium thiosulphate‚ sodium citrate‚ ox bile‚ sucrose‚ sodium chloride‚ ferric citrate‚ Bromothymol blue/ thymol blue and agar. Sodium thiosulphate‚ sodium citrate and ox bile are the components
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of common household spices/herbs such as cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic‚ on the zone of inhibition in the Staphylococcus epidermis covered nutrient agar? Hypothesis: If 3 teaspoons of chopped cinnamon‚ cloves‚ mustard‚ ginger‚ and garlic steep in half a cup of boiling water for 15 hours and then placed in nutrient agar covered with Staphylococcus epidermis (gram positive) bacteria for 24 hours (in an incubator)‚ garlic will have the strongest antimicrobial effect. This is because
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Bacteria will be grown in an agar broth containing .01%‚ .1%‚ .5% and 10% antibacterial soap. To fully understand the subject‚ information on everything necessary to conduct this experiment and comprehend the results was researched. Once the experiment is completed a conclusion will be able to be made as to whether or not bacteria can become resistant to antibacterial soaps The procedure for the experiment is as follows. Ecoli bacteria will be grown in agar broth with .01%‚ .1%‚ .5% and 10%
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using a glass tube and an agar-water gel. In glass tube test‚ two cotton plugs submerse with two different substances – hydrochloric acid (HCL) and ammonium hydroxide (NH4OH) on the other end of the tube. By their molecular weight‚ NH4OH has a small molecular weight that diffuses at a faster rate. While the formation of a white cloud was first occurred at the end of the tube containing HCL with a large molecular weight. In agar-water gel test‚ using agar plate and agar-water gel‚ 2 different substrate
Free Diffusion Molecular diffusion Potassium permanganate
Project 1: Antimicrobial Activity of Soil Isolates John Franklin Farrar Department of Biology and Microbiology and Biology Address BOX 22750 BOWEN HALL‚ RALEIGH‚ NC‚ 27607 Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining
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